Differentiation of human pluripotent stem cells to cardiomyocytes under defined conditions

Cathelijne W. Van Den Berg, David A. Elliott, Stefan R. Braam, Christine L Mummery, Richard P. Davis

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

30 Citations (Scopus)

Abstract

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can differentiate to cardiomyocytes in vitro, offering unique opportunities to investigate cardiac development and disease as well as providing a platform to perform drug and toxicity tests. Initial cardiac differentiation methods were based on either inductive co-culture or aggregation as embryoid bodies, often in the presence of fetal calf serum. More recently, monolayer differentiation protocols have evolved as feasible alternatives and are often performed in completely defined culture medium and substrates. Thus, our ability to efficiently and reproducibly generate cardiomyocytes from multiple different hESC and hiPSC lines has improved significantly. We have developed a directed differentiation monolayer protocol that can be used to generate cultures comprising ~50 % cardiomyocytes, in which both the culture of the undifferentiated human pluripotent stem cells (hPSCs) and the differentiation procedure itself are defined and serum-free. The differentiation method is also effective for hPSCs maintained in other culture systems. In this chapter, we outline the differentiation protocol and describe methods to assess cardiac differentiation efficiency as well as to identify and quantify the yield of cardiomyocytes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages163-180
Number of pages18
Volume1353
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1353
ISSN (Print)10643745

Keywords

  • Cardiac differentiation
  • Cardiomyocyte characterization
  • Human embryonic stem cells
  • Human induced pluripotent stem cells

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