Differentiation of Brucella ovis from Brucella abortus by gas-liquid chromatographic analysis of cellular fatty acids

P. J. Coloe, A. J. Sinclair, J. F. Slattery, D. Burke

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Abstract

The cellular fatty acid composition of Brucella ovis and Brucella abortus strains was determined by gas-liquid chromatography. Both species were characterized by the presence of fatty acids 16:0, 17:0, 17:0 cyclopropane, 18:0, 18:1, and 19:0 cyclopropane; B, ovis also contained some 15:0. There were differences in the relative proportions of the fatty acids present, and it was possible to differentiate B. ovis from B. abortus on the basis of the absence of 15:0, lower concentrations of 17:0 and 18:1, and higher concentrations of 19:0 cyclopropane in B. abortus. The data indicate that analysis of cellular fatty acid composition by gasliquid chromatography can be used for the identification of B. ovis and its differentiation from B. abortus.

Original languageEnglish
Pages (from-to)896-898
Number of pages3
JournalJournal of Clinical Microbiology
Volume19
Issue number6
Publication statusPublished - 1 Jan 1984
Externally publishedYes

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