Cross-presentation is the mechanism by which exogenous Ag is processed for recognition by CD8+ T cells. Murine CD8α+ DCs are specialized at cross-presenting soluble and cellular Ag, but in humans this process is poorly characterized. In this study, we examined uptake and cross-presentation of soluble and cellular Ag by human blood CD141+ DCs, the human equivalent of mouse CD8α+ DCs, and compared them with human monocyte-derived DCs (MoDCs) and blood CD1c+ DC subsets. MoDCs were superior in their capacity to internalize and cross-present soluble protein whereas CD141+ DCs were more efficient at ingesting and cross-presenting cellular Ag. Whilst cross-presentation by CD1c+ DCs and CD141+ DCs was dependent on the proteasome, and hence cytosolic translocation, cross-presentation by MoDCs was not. Inhibition of endosomal acidification enhanced cross-presentation by CD1c+ DCs and MoDCs but not by CD141+ DCs. These data demonstrate that CD1c+ DCs, CD141+ DCs, and MoDCs are capable of cross-presentation; however, they do so via different mechanisms. Moreover, they demonstrate that human CD141+ DCs, like their murine CD8α+ DC counterparts, are specialized at cross-presenting cellular Ag, most likely mediated by an enhanced capacity to ingest cellular Ag combined with subtle changes in lysosomal pH during Ag processing and use of the cytosolic pathway.
- antigen processing
- human dendritic cells
Chiang, M-C., Tullett, K. M.
, Lee, Y. S., Idris, A., Ding, Y., McDonald, K. J., Kassianos, A., Leal Rojas, I. M., Jeet, V., Lahoud, M.
, & Radford, K. J. (2016). Differential uptake and cross-presentation of soluble and necrotic cell antigen by human DC subsets
. European Journal of Immunology
(2), 329-339. https://doi.org/10.1002/eji.201546023