Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro

Mari Norimatsu, James Harris, Victoria Chance, Gordon Dougan, Christopher J. Howard, Bernardo Villarreal-Ramos

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Exposing bovine dendritic cells (DC) and macrophages (MΠ) to Salmonella typhimurium at a ratio of 1 cell to 10 bacteria had a cytotoxic effect that was not evident with a ratio of 1000 cells to 1 bacterium. This lower dose was considered to mimic more closely the in vivo situation and a comparison was made with this model of the consequences of infection for MΠ and DC. DC infected with S. typhimurium up-regulated cell surface expression of major histocompatibility class I (MHC-I), MHC-II, CD40, CD80 and CD86. In contrast, infected MΠ did not exhibit detectable changes in expression of cell surface molecules, except for a marginal increase in CD40. mRNA transcription for tumour necrosis factor-α, interleukin (IL)-1β, IL-6 and inducible nitric oxide synthase was up-regulated in both infected DC and infected MΠ, although mRNA transcription for granulocyte-macrophage colony-stimulating factor and IL-12p40 was up-regulated only in infected DC and for IL-10 was only in infected MΠ. Infected DC had an increased ability to stimulate both allogeneic and antigen-specific T-cell responses compared to non-infected controls. In contrast, infected MΠ showed an increased ability to induce allogeneic responses but this was less than seen for DC and no enhancement of ability to induce antigen-specific T cell responses was seen. Thus, in a low-dose infection model that does not result in the cytotoxicity of a substantial percentage of antigen presenting cells, bovine MΠ and DC respond differently to infection with S. typhimurium and this could have important implications for the development of the immune response.

Original languageEnglish
Pages (from-to)55-61
Number of pages7
JournalImmunology
Volume108
Issue number1
DOIs
Publication statusPublished - 2003
Externally publishedYes

Cite this

Norimatsu, Mari ; Harris, James ; Chance, Victoria ; Dougan, Gordon ; Howard, Christopher J. ; Villarreal-Ramos, Bernardo. / Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro. In: Immunology. 2003 ; Vol. 108, No. 1. pp. 55-61.
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abstract = "Exposing bovine dendritic cells (DC) and macrophages (MΠ) to Salmonella typhimurium at a ratio of 1 cell to 10 bacteria had a cytotoxic effect that was not evident with a ratio of 1000 cells to 1 bacterium. This lower dose was considered to mimic more closely the in vivo situation and a comparison was made with this model of the consequences of infection for MΠ and DC. DC infected with S. typhimurium up-regulated cell surface expression of major histocompatibility class I (MHC-I), MHC-II, CD40, CD80 and CD86. In contrast, infected MΠ did not exhibit detectable changes in expression of cell surface molecules, except for a marginal increase in CD40. mRNA transcription for tumour necrosis factor-α, interleukin (IL)-1β, IL-6 and inducible nitric oxide synthase was up-regulated in both infected DC and infected MΠ, although mRNA transcription for granulocyte-macrophage colony-stimulating factor and IL-12p40 was up-regulated only in infected DC and for IL-10 was only in infected MΠ. Infected DC had an increased ability to stimulate both allogeneic and antigen-specific T-cell responses compared to non-infected controls. In contrast, infected MΠ showed an increased ability to induce allogeneic responses but this was less than seen for DC and no enhancement of ability to induce antigen-specific T cell responses was seen. Thus, in a low-dose infection model that does not result in the cytotoxicity of a substantial percentage of antigen presenting cells, bovine MΠ and DC respond differently to infection with S. typhimurium and this could have important implications for the development of the immune response.",
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Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro. / Norimatsu, Mari; Harris, James; Chance, Victoria; Dougan, Gordon; Howard, Christopher J.; Villarreal-Ramos, Bernardo.

In: Immunology, Vol. 108, No. 1, 2003, p. 55-61.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro

AU - Norimatsu, Mari

AU - Harris, James

AU - Chance, Victoria

AU - Dougan, Gordon

AU - Howard, Christopher J.

AU - Villarreal-Ramos, Bernardo

PY - 2003

Y1 - 2003

N2 - Exposing bovine dendritic cells (DC) and macrophages (MΠ) to Salmonella typhimurium at a ratio of 1 cell to 10 bacteria had a cytotoxic effect that was not evident with a ratio of 1000 cells to 1 bacterium. This lower dose was considered to mimic more closely the in vivo situation and a comparison was made with this model of the consequences of infection for MΠ and DC. DC infected with S. typhimurium up-regulated cell surface expression of major histocompatibility class I (MHC-I), MHC-II, CD40, CD80 and CD86. In contrast, infected MΠ did not exhibit detectable changes in expression of cell surface molecules, except for a marginal increase in CD40. mRNA transcription for tumour necrosis factor-α, interleukin (IL)-1β, IL-6 and inducible nitric oxide synthase was up-regulated in both infected DC and infected MΠ, although mRNA transcription for granulocyte-macrophage colony-stimulating factor and IL-12p40 was up-regulated only in infected DC and for IL-10 was only in infected MΠ. Infected DC had an increased ability to stimulate both allogeneic and antigen-specific T-cell responses compared to non-infected controls. In contrast, infected MΠ showed an increased ability to induce allogeneic responses but this was less than seen for DC and no enhancement of ability to induce antigen-specific T cell responses was seen. Thus, in a low-dose infection model that does not result in the cytotoxicity of a substantial percentage of antigen presenting cells, bovine MΠ and DC respond differently to infection with S. typhimurium and this could have important implications for the development of the immune response.

AB - Exposing bovine dendritic cells (DC) and macrophages (MΠ) to Salmonella typhimurium at a ratio of 1 cell to 10 bacteria had a cytotoxic effect that was not evident with a ratio of 1000 cells to 1 bacterium. This lower dose was considered to mimic more closely the in vivo situation and a comparison was made with this model of the consequences of infection for MΠ and DC. DC infected with S. typhimurium up-regulated cell surface expression of major histocompatibility class I (MHC-I), MHC-II, CD40, CD80 and CD86. In contrast, infected MΠ did not exhibit detectable changes in expression of cell surface molecules, except for a marginal increase in CD40. mRNA transcription for tumour necrosis factor-α, interleukin (IL)-1β, IL-6 and inducible nitric oxide synthase was up-regulated in both infected DC and infected MΠ, although mRNA transcription for granulocyte-macrophage colony-stimulating factor and IL-12p40 was up-regulated only in infected DC and for IL-10 was only in infected MΠ. Infected DC had an increased ability to stimulate both allogeneic and antigen-specific T-cell responses compared to non-infected controls. In contrast, infected MΠ showed an increased ability to induce allogeneic responses but this was less than seen for DC and no enhancement of ability to induce antigen-specific T cell responses was seen. Thus, in a low-dose infection model that does not result in the cytotoxicity of a substantial percentage of antigen presenting cells, bovine MΠ and DC respond differently to infection with S. typhimurium and this could have important implications for the development of the immune response.

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DO - 10.1046/j.1365-2567.2003.01557.x

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JF - Immunology

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