Differential regulation of SF-1-cofactor interactions

Johan Lund, Bente Borud, Gunnar Mellgren, Reidun Aesoey, Tuyen Hoang, Anne L Jacob, Marit Bakke

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8 Citations (Scopus)

Abstract

The orphan nuclear receptor steroidogenic factor-1 (SF-1) plays pivotal roles in the development and function of steroidogenic organs. Here we describe the differential effect of protein kinase A (PKA) on coregulation of SF-1 dependent transcription by two p160 family members, p300/CBP co-integrator-associated protein (p/CIP) and transcription intermediary factor-2 (TIF2). Thus, whereas p/CIP-stimulated SF-1 dependent transcription is further potentiated by PKA, we show that activation of PKA leads to selective downregulation of TIF2 protein and a subsequent repression of TIF2 coactivator function. Using a yeast two-hybrid screen we also identified a novel zinc finger containing protein, which interacts with SF-1 via the AF-2 domain.
Original languageEnglish
Pages (from-to)505 - 513
Number of pages9
JournalEndocrine Research
Volume28
Issue number4
Publication statusPublished - 2002
Externally publishedYes

Cite this

Lund, J., Borud, B., Mellgren, G., Aesoey, R., Hoang, T., Jacob, A. L., & Bakke, M. (2002). Differential regulation of SF-1-cofactor interactions. Endocrine Research, 28(4), 505 - 513.