Differential phenotypes of tissue-infiltrating T cells during angiotensin II-induced hypertension in mice

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Hypertension remains the leading risk factor for cardiovascular disease (CVD). Experimental hypertension is associated with increased T cell infiltration into blood pressure-controlling organs, such as the aorta and kidney; importantly in absence of T cells of the adaptive immune system, experimental hypertension is significantly blunted. However, the function and phenotype of these T cell infiltrates remains speculative and undefined in the setting of hypertension. The current study compared T cell-derived cytokine and reactive oxygen species (ROS) production from normotensive and hypertensive mice. Splenic, blood, aortic, kidney and brain T cells were isolated from C57BL/6J mice following 14-day vehicle or angiotensin (Ang) II (0.7 mg/kg/day, s.c.) infusion. T cell infiltration was increased in aorta, kidney and brain from hypertensive mice. Cytokine analysis in stimulated T cells indicated an overall Th1 pro-inflammatory phenotype, but a similar proportion (flow cytometry) and quantity (cytometric bead array) of IFN-gamma, TNF-alpha, IL-4 and IL-17 between vehicle- and Ang II- treated groups. Strikingly, elevated T cell-derived production of a chemokine, chemokine C-C motif ligand 2 (CCL2), was observed in aorta ( approximately 6-fold) and kidney in response to Ang II, but not in brain, spleen or blood. Moreover, T cell-derived ROS production in aorta was elevated approximately 3 -fold in Ang II-treated mice (n = 7; P
Original languageEnglish
Article numbere114895
Number of pages21
JournalPLoS ONE
Volume9
Issue number12
DOIs
Publication statusPublished - 2014

Cite this

@article{10b7da57bd2046429a926be8d5b3de96,
title = "Differential phenotypes of tissue-infiltrating T cells during angiotensin II-induced hypertension in mice",
abstract = "Hypertension remains the leading risk factor for cardiovascular disease (CVD). Experimental hypertension is associated with increased T cell infiltration into blood pressure-controlling organs, such as the aorta and kidney; importantly in absence of T cells of the adaptive immune system, experimental hypertension is significantly blunted. However, the function and phenotype of these T cell infiltrates remains speculative and undefined in the setting of hypertension. The current study compared T cell-derived cytokine and reactive oxygen species (ROS) production from normotensive and hypertensive mice. Splenic, blood, aortic, kidney and brain T cells were isolated from C57BL/6J mice following 14-day vehicle or angiotensin (Ang) II (0.7 mg/kg/day, s.c.) infusion. T cell infiltration was increased in aorta, kidney and brain from hypertensive mice. Cytokine analysis in stimulated T cells indicated an overall Th1 pro-inflammatory phenotype, but a similar proportion (flow cytometry) and quantity (cytometric bead array) of IFN-gamma, TNF-alpha, IL-4 and IL-17 between vehicle- and Ang II- treated groups. Strikingly, elevated T cell-derived production of a chemokine, chemokine C-C motif ligand 2 (CCL2), was observed in aorta ( approximately 6-fold) and kidney in response to Ang II, but not in brain, spleen or blood. Moreover, T cell-derived ROS production in aorta was elevated approximately 3 -fold in Ang II-treated mice (n = 7; P",
author = "Zihui Wei and Iresha Spizzo and Henry Diep and Drummond, {Grant R} and Widdop, {Robert E} and Antony Vinh",
year = "2014",
doi = "10.1371/journal.pone.0114895",
language = "English",
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journal = "PLoS ONE",
issn = "1932-6203",
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Differential phenotypes of tissue-infiltrating T cells during angiotensin II-induced hypertension in mice. / Wei, Zihui; Spizzo, Iresha; Diep, Henry; Drummond, Grant R; Widdop, Robert E; Vinh, Antony.

In: PLoS ONE, Vol. 9, No. 12, e114895, 2014.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Vinh, Antony

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