Differential expression of the duffy antigen receptor for chemokines according to RBC age and Fy genotype

Ian J. Woolley, K. A. Hotmire, R. M. Sramkoski, P. A. Zimmerman, J. W. Kazura

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38 Citations (Scopus)

Abstract

BACKGROUND: The Duffy (Fy) blood group (also known as Duffy antigen receptor for chemokines, or DARC) may be involved in regulation of the level of circulating proinflammatory chemokines, and it is an obligatory receptor on RBCs for the human malaria parasite Plasmodium vivax. STUDY DESIGN AND METHODS: Because quantification of Fy expression by using RBCs of various ages will not detect acute changes associated with inflammatory states, and because P. vivax exclusively invades reticulocytes, a flow cytometric method was developed to measure the level of surface expression of Fy. Reticulocytes and mature RBCs from persons with different genotypes (GATA-1 T→C promoter mutation at nt -46; FY(*)A and FY(*)B in the ORF) were used. RESULTS: Expression of the Fy6 epitope, which is required for P. vivax invasion, was 49 ± 19 percent higher on reticulocytes than on mature RBCs, regardless of donor genotype (p

Original languageEnglish
Pages (from-to)949-953
Number of pages5
JournalTransfusion
Volume40
Issue number8
DOIs
Publication statusPublished - 2000
Externally publishedYes

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