Abstract
The multidrug resistance (mdr1) gene family encodes the efflux transporter P-glycoprotein (P-gp) which contributes to the functionality of the blood-brain barrier. We have characterised the regional expression of mdr1 genes in naïve rat brain. Adult male Sprague-Dawley rats (n=6) were sacrificed and their brains rapidly removed. Seven distinct anatomical regions were isolated by microdissection and the expression of mdr1a and mdr1b determined by quantitative reverse-transcriptase polymerase chain reaction. The mdr1a isoform was expressed in all brain regions investigated, while mdr1b was expressed to a quantifiable degree in hippocampus alone. These findings reveal a differential expression of mdr1 genes in normal rodent brain tissue and suggest that P-gp may afford a broader protection of the hippocampus than other brain structures.
| Original language | English |
|---|---|
| Pages (from-to) | 33-36 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 339 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 13 Mar 2003 |
| Externally published | Yes |
Keywords
- Brain
- Multidrug resistance
- P-glycoprotein
- Quantitative reverse-transcriptase polymerase chain reaction
- Rat
Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver