Differential expression of multidrug resistance genes in naïve rat brain

Patrick Kwan; Graeme J. Sills; Elaine Butler; Timothy W. Gant; Martin J. Brodie

doi:10.1016/S0304-3940(02)01453-2

Differential expression of multidrug resistance genes in naïve rat brain

Patrick Kwan, Graeme J. Sills, Elaine Butler, Timothy W. Gant, Martin J. Brodie

Research output: Contribution to journal › Article › Research › peer-review

35 Citations (Scopus)

Abstract

The multidrug resistance (mdr1) gene family encodes the efflux transporter P-glycoprotein (P-gp) which contributes to the functionality of the blood-brain barrier. We have characterised the regional expression of mdr1 genes in naïve rat brain. Adult male Sprague-Dawley rats (n=6) were sacrificed and their brains rapidly removed. Seven distinct anatomical regions were isolated by microdissection and the expression of mdr1a and mdr1b determined by quantitative reverse-transcriptase polymerase chain reaction. The mdr1a isoform was expressed in all brain regions investigated, while mdr1b was expressed to a quantifiable degree in hippocampus alone. These findings reveal a differential expression of mdr1 genes in normal rodent brain tissue and suggest that P-gp may afford a broader protection of the hippocampus than other brain structures.

Original language	English
Pages (from-to)	33-36
Number of pages	4
Journal	Neuroscience Letters
Volume	339
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(02)01453-2
Publication status	Published - 13 Mar 2003
Externally published	Yes

Keywords

Brain
Multidrug resistance
P-glycoprotein
Quantitative reverse-transcriptase polymerase chain reaction
Rat

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10.1016/S0304-3940(02)01453-2

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AU - Kwan, Patrick

AU - Sills, Graeme J.

AU - Butler, Elaine

AU - Gant, Timothy W.

AU - Brodie, Martin J.

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AB - The multidrug resistance (mdr1) gene family encodes the efflux transporter P-glycoprotein (P-gp) which contributes to the functionality of the blood-brain barrier. We have characterised the regional expression of mdr1 genes in naïve rat brain. Adult male Sprague-Dawley rats (n=6) were sacrificed and their brains rapidly removed. Seven distinct anatomical regions were isolated by microdissection and the expression of mdr1a and mdr1b determined by quantitative reverse-transcriptase polymerase chain reaction. The mdr1a isoform was expressed in all brain regions investigated, while mdr1b was expressed to a quantifiable degree in hippocampus alone. These findings reveal a differential expression of mdr1 genes in normal rodent brain tissue and suggest that P-gp may afford a broader protection of the hippocampus than other brain structures.

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KW - Multidrug resistance

KW - P-glycoprotein

KW - Quantitative reverse-transcriptase polymerase chain reaction

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Differential expression of multidrug resistance genes in naïve rat brain

Abstract

Keywords

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