Differential effects of allergen challenge on large and small airway reactivity in mice

Chantal Donovan, Simon G. Royce, James Esposito, Jenny Tran, Zaridatul Aini Ibrahim, Mimi L K Tang, Simon Bailey, Jane E. Bourke

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

The relative contributions of large and small airways to hyperresponsiveness in asthma have yet to be fully assessed. This study used a mouse model of chronic allergic airways disease to induce inflammation and remodelling and determine whether in vivo hyperresponsiveness to methacholine is consistent with in vitro reactivity of trachea and small airways. Balb/C mice were sensitised (days 0, 14) and challenged (3 times/week, 6 weeks) with ovalbumin. Airway reactivity was compared with saline-challenged controls in vivo assessing whole lung resistance, and in vitro measuring the force of tracheal contraction and the magnitude/rate of small airway narrowing within lung slices. Increased airway inflammation, epithelial remodelling and fibrosis were evident following allergen challenge. In vivo hyperresponsiveness to methacholine was maintained in isolated trachea. In contrast, methacholine induced slower narrowing, with reduced potency in small airways compared to controls. In vitro incubation with IL-1/TNFα did not alter reactivity. The hyporesponsiveness to methacholine in small airways within lung slices following chronic ovalbumin challenge was unexpected, given hyperresponsiveness to the same agonist both in vivo and in vitro in tracheal preparations. This finding may reflect the altered interactions of small airways with surrounding parenchymal tissue after allergen challenge to oppose airway narrowing and closure.

Original languageEnglish
Article numbere74101
Pages (from-to)1-8
Number of pages8
JournalPLoS ONE
Volume8
Issue number9
DOIs
Publication statusPublished - 6 Sep 2013
Externally publishedYes

Cite this

Donovan, Chantal ; Royce, Simon G. ; Esposito, James ; Tran, Jenny ; Ibrahim, Zaridatul Aini ; Tang, Mimi L K ; Bailey, Simon ; Bourke, Jane E. / Differential effects of allergen challenge on large and small airway reactivity in mice. In: PLoS ONE. 2013 ; Vol. 8, No. 9. pp. 1-8.
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abstract = "The relative contributions of large and small airways to hyperresponsiveness in asthma have yet to be fully assessed. This study used a mouse model of chronic allergic airways disease to induce inflammation and remodelling and determine whether in vivo hyperresponsiveness to methacholine is consistent with in vitro reactivity of trachea and small airways. Balb/C mice were sensitised (days 0, 14) and challenged (3 times/week, 6 weeks) with ovalbumin. Airway reactivity was compared with saline-challenged controls in vivo assessing whole lung resistance, and in vitro measuring the force of tracheal contraction and the magnitude/rate of small airway narrowing within lung slices. Increased airway inflammation, epithelial remodelling and fibrosis were evident following allergen challenge. In vivo hyperresponsiveness to methacholine was maintained in isolated trachea. In contrast, methacholine induced slower narrowing, with reduced potency in small airways compared to controls. In vitro incubation with IL-1/TNFα did not alter reactivity. The hyporesponsiveness to methacholine in small airways within lung slices following chronic ovalbumin challenge was unexpected, given hyperresponsiveness to the same agonist both in vivo and in vitro in tracheal preparations. This finding may reflect the altered interactions of small airways with surrounding parenchymal tissue after allergen challenge to oppose airway narrowing and closure.",
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Donovan, C, Royce, SG, Esposito, J, Tran, J, Ibrahim, ZA, Tang, MLK, Bailey, S & Bourke, JE 2013, 'Differential effects of allergen challenge on large and small airway reactivity in mice', PLoS ONE, vol. 8, no. 9, e74101, pp. 1-8. https://doi.org/10.1371/journal.pone.0074101

Differential effects of allergen challenge on large and small airway reactivity in mice. / Donovan, Chantal; Royce, Simon G.; Esposito, James; Tran, Jenny; Ibrahim, Zaridatul Aini; Tang, Mimi L K; Bailey, Simon; Bourke, Jane E.

In: PLoS ONE, Vol. 8, No. 9, e74101, 06.09.2013, p. 1-8.

Research output: Contribution to journalArticleResearchpeer-review

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