Differential distribution of mRNA for the α- and β-subunits of chorionic gonadotrophin in the implantation stage blastocyst of the marmoset monkey

A. Lopata, J. Berka, A. Simula, R. Norman, T. Otani

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We studied the expression of mRNA encoding the a- and β-subunts of marmoset chrrionic gonadotrophin (mCG) in implantation stage blastocysts and in a tropho-blastic cell line derived from such blastocysts. In this investigation in situ hybridization was carried out using digoxygenin-labelled riboprobes to localize the subunit transcripts. The trophoblastic cell line, known to secrete bioactive mCG, was used as a positive control. Marmoset uterine embryos were cultured to hatched blastocysts and following growth on Matrigel or plastic were processed for in situ hybridization at developmental stages ranging from 13-15 days post-conception. In serial sections mCG-β mRNA was detected mainly in polar, trophoblast. The mRNA for mCG-a was expressed more uniformly in polar and mural trophoblast. Transcripts for the β-subunit were not expressed, or present as weak signals, in the inner cell mass (ICM) and endoderm. However, low levels of mRNA for mCG-a were detected in the ICM and visceral endoderm. We have concluded that mRNA for mCG-β was primarily localized to patches of syncytiotrophoblast at the embryonic pole and sparsely distributed in mural trophoblast, while the transcripts for mCG-a were distributed more uniformly in differentiating cytotrophoblast and syncytium, and at much lower levels in ICM and early endoderm.

Original languageEnglish
Pages (from-to)335-346
Number of pages12
Issue number4
Publication statusPublished - 1 Jan 1995

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