Differential binding of human interferon-α subtypes to receptors on lymphoblastoid cells

Gregory E. Hannigan, Dirk R. Gewert, Eleanor N. Fish, Stanley E. Read, Bryan R.G. Williams

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Highly purified human interferon-α subtype A (HuIFNαA) was iodinated for use in direct ligand binding studies on human lymphoblastoid (Daudi) cells. Unlabelled preparations of HuIFNα subtypes A, C, D, and hybrid molecules AD (Bgl II), AD (Pvu II), and DA (Bgl II) showed different responses in competition experiments with labelled αA probe. Specifically, IFNs αD and αDA were unable to displace the probe, whereas IFNS αA, αC, and the hybrid αADs showed similar competition curves. These results support a two-idiotope model of IFN recognition by its receptor. IFN effects on [3H]-thymidine incorporation and cell growth (long term effects) did not reflect the apparent affinities of HuIFNα subtypes for cell surface receptor.

Original languageEnglish
Pages (from-to)537-544
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 27 Jan 1983
Externally publishedYes

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