Differential binding of human interferon-α subtypes to receptors on lymphoblastoid cells

Gregory E. Hannigan; Dirk R. Gewert; Eleanor N. Fish; Stanley E. Read; Bryan R.G. Williams

doi:10.1016/0006-291X(83)91183-X

Differential binding of human interferon-α subtypes to receptors on lymphoblastoid cells

Gregory E. Hannigan, Dirk R. Gewert, Eleanor N. Fish, Stanley E. Read, Bryan R.G. Williams

Research output: Contribution to journal › Article › Research › peer-review

35 Citations (Scopus)

Abstract

Highly purified human interferon-α subtype A (HuIFNαA) was iodinated for use in direct ligand binding studies on human lymphoblastoid (Daudi) cells. Unlabelled preparations of HuIFNα subtypes A, C, D, and hybrid molecules AD (Bgl II), AD (Pvu II), and DA (Bgl II) showed different responses in competition experiments with labelled αA probe. Specifically, IFNs αD and αDA were unable to displace the probe, whereas IFNS αA, αC, and the hybrid αADs showed similar competition curves. These results support a two-idiotope model of IFN recognition by its receptor. IFN effects on [³H]-thymidine incorporation and cell growth (long term effects) did not reflect the apparent affinities of HuIFNα subtypes for cell surface receptor.

Original language	English
Pages (from-to)	537-544
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	110
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(83)91183-X
Publication status	Published - 27 Jan 1983
Externally published	Yes

Access to Document

10.1016/0006-291X(83)91183-X

Cite this

@article{e5511bdd488c4feda027a2a4fc03deba,

title = "Differential binding of human interferon-α subtypes to receptors on lymphoblastoid cells",

abstract = "Highly purified human interferon-α subtype A (HuIFNαA) was iodinated for use in direct ligand binding studies on human lymphoblastoid (Daudi) cells. Unlabelled preparations of HuIFNα subtypes A, C, D, and hybrid molecules AD (Bgl II), AD (Pvu II), and DA (Bgl II) showed different responses in competition experiments with labelled αA probe. Specifically, IFNs αD and αDA were unable to displace the probe, whereas IFNS αA, αC, and the hybrid αADs showed similar competition curves. These results support a two-idiotope model of IFN recognition by its receptor. IFN effects on [3H]-thymidine incorporation and cell growth (long term effects) did not reflect the apparent affinities of HuIFNα subtypes for cell surface receptor.",

author = "Hannigan, {Gregory E.} and Gewert, {Dirk R.} and Fish, {Eleanor N.} and Read, {Stanley E.} and Williams, {Bryan R.G.}",

year = "1983",

month = jan,

day = "27",

doi = "10.1016/0006-291X(83)91183-X",

language = "English",

volume = "110",

pages = "537--544",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Differential binding of human interferon-α subtypes to receptors on lymphoblastoid cells

AU - Hannigan, Gregory E.

AU - Gewert, Dirk R.

AU - Fish, Eleanor N.

AU - Read, Stanley E.

AU - Williams, Bryan R.G.

PY - 1983/1/27

Y1 - 1983/1/27

N2 - Highly purified human interferon-α subtype A (HuIFNαA) was iodinated for use in direct ligand binding studies on human lymphoblastoid (Daudi) cells. Unlabelled preparations of HuIFNα subtypes A, C, D, and hybrid molecules AD (Bgl II), AD (Pvu II), and DA (Bgl II) showed different responses in competition experiments with labelled αA probe. Specifically, IFNs αD and αDA were unable to displace the probe, whereas IFNS αA, αC, and the hybrid αADs showed similar competition curves. These results support a two-idiotope model of IFN recognition by its receptor. IFN effects on [3H]-thymidine incorporation and cell growth (long term effects) did not reflect the apparent affinities of HuIFNα subtypes for cell surface receptor.

AB - Highly purified human interferon-α subtype A (HuIFNαA) was iodinated for use in direct ligand binding studies on human lymphoblastoid (Daudi) cells. Unlabelled preparations of HuIFNα subtypes A, C, D, and hybrid molecules AD (Bgl II), AD (Pvu II), and DA (Bgl II) showed different responses in competition experiments with labelled αA probe. Specifically, IFNs αD and αDA were unable to displace the probe, whereas IFNS αA, αC, and the hybrid αADs showed similar competition curves. These results support a two-idiotope model of IFN recognition by its receptor. IFN effects on [3H]-thymidine incorporation and cell growth (long term effects) did not reflect the apparent affinities of HuIFNα subtypes for cell surface receptor.

UR - http://www.scopus.com/inward/record.url?scp=0020677674&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(83)91183-X

DO - 10.1016/0006-291X(83)91183-X

M3 - Article

C2 - 6301456

AN - SCOPUS:0020677674

VL - 110

SP - 537

EP - 544

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

Differential binding of human interferon-α subtypes to receptors on lymphoblastoid cells

Abstract

Access to Document

Other files and links

Cite this