Abstract
Patterns of protein synthesis by genital skin fibroblasts from three unrelated normal individuals and three unrelated patients with complete testicular feminization were compared by two-dimensional gel electrophoresis. Cell lines were maintained in monolayer culture and pulse labeled with [35S]methionine. Cells were lysed in 9 M urea, and aliquots of 20 μl subjected to isoelectric focussing and polyacrylamide gel electrophoresis followed by autoradiography. Gels of control fibroblasts showed two proteins (mol wt ~ 45,000, ~ 85,000; pK(i) ~ 5.0) markedly more prominent than on gels from affected fibroblasts. This pattern was unaltered by prior exposure to dihydrotestosterone, suggesting differences in constitutive proteins of the fibroblast cells. Parallel studies demonstrated a marked reduction in the ability of fibroblasts from patients with complete testicular feminization to bind androgens in vitro compared with those of normal individuals. The relationship between these proteins, androgen receptors, and androgen insensitivity requires further investigation.
| Original language | English |
|---|---|
| Pages (from-to) | 99-103 |
| Number of pages | 5 |
| Journal | Journal of Clinical Investigation |
| Volume | 69 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1982 |
| Externally published | Yes |
Cite this
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Differences in proteins synthesized by fibroblasts from normal individuals and patients with complete testicular feminization. / Risbridger, G. P.; Khalid, B. A.K.; Warne, G. L.; Funder, J. W.
In: Journal of Clinical Investigation, Vol. 69, No. 1, 1982, p. 99-103.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Differences in proteins synthesized by fibroblasts from normal individuals and patients with complete testicular feminization
AU - Risbridger, G. P.
AU - Khalid, B. A.K.
AU - Warne, G. L.
AU - Funder, J. W.
PY - 1982
Y1 - 1982
N2 - Patterns of protein synthesis by genital skin fibroblasts from three unrelated normal individuals and three unrelated patients with complete testicular feminization were compared by two-dimensional gel electrophoresis. Cell lines were maintained in monolayer culture and pulse labeled with [35S]methionine. Cells were lysed in 9 M urea, and aliquots of 20 μl subjected to isoelectric focussing and polyacrylamide gel electrophoresis followed by autoradiography. Gels of control fibroblasts showed two proteins (mol wt ~ 45,000, ~ 85,000; pK(i) ~ 5.0) markedly more prominent than on gels from affected fibroblasts. This pattern was unaltered by prior exposure to dihydrotestosterone, suggesting differences in constitutive proteins of the fibroblast cells. Parallel studies demonstrated a marked reduction in the ability of fibroblasts from patients with complete testicular feminization to bind androgens in vitro compared with those of normal individuals. The relationship between these proteins, androgen receptors, and androgen insensitivity requires further investigation.
AB - Patterns of protein synthesis by genital skin fibroblasts from three unrelated normal individuals and three unrelated patients with complete testicular feminization were compared by two-dimensional gel electrophoresis. Cell lines were maintained in monolayer culture and pulse labeled with [35S]methionine. Cells were lysed in 9 M urea, and aliquots of 20 μl subjected to isoelectric focussing and polyacrylamide gel electrophoresis followed by autoradiography. Gels of control fibroblasts showed two proteins (mol wt ~ 45,000, ~ 85,000; pK(i) ~ 5.0) markedly more prominent than on gels from affected fibroblasts. This pattern was unaltered by prior exposure to dihydrotestosterone, suggesting differences in constitutive proteins of the fibroblast cells. Parallel studies demonstrated a marked reduction in the ability of fibroblasts from patients with complete testicular feminization to bind androgens in vitro compared with those of normal individuals. The relationship between these proteins, androgen receptors, and androgen insensitivity requires further investigation.
UR - http://www.scopus.com/inward/record.url?scp=0020059775&partnerID=8YFLogxK
U2 - 10.1172/JCI110446
DO - 10.1172/JCI110446
M3 - Article
VL - 69
SP - 99
EP - 103
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
SN - 0021-9738
IS - 1
ER -