Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice

Angélica T. Vieira, Izabela Galvão, Laurence M. Macia, Erica M. Sernaglia, Marco Aurélio R. Vinolo, Cristiana Couto Garcia, Luciana Padua Tavares, Flávio A. Amaral, Lirlândia P. Sousa, Flaviano S Martins, Charles R. Mackay, Mauro Martin Teixeira

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26 Citations (Scopus)

Abstract

Gout is a disease characterized by the deposition of monosodium urate (MSU) crystals in the joints. Continuous gout episodes may lead to unresolved inflammatory responses and tissue damage. We investigated the effects of a high-fiber diet and acetate, a short-chain fatty acid (SCFA) resulting from the metabolism of fiber by gut microbiota, on the inflammatory response in an experimental model of gout in mice. Injection of MSU crystals into the knee joint of mice induced neutrophil influx and inflammatory hypernociception. The onset of inflammatory response induced by MSU crystals was not altered in animals given a high-fiber diet, but the highfiber diet induced faster resolution of the inflammatory response. Similar results were obtained in animals given the SCFA acetate. Acetate was effective, even when given after injection of MSU crystals at the peak of the inflammatory response and induced caspasedependent apoptosis of neutrophils that accounted for the resolution of inflammation. Resolution of neutrophilic inflammation was associated with decreased NF-κB activity and enhanced production of anti-inflammatory mediators, including IL-10, TGF-β, and annexin A1. Acetate treatment or intake of a high-fiber diet enhanced efferocytosis, an effect also observed in vitro with neutrophils treated with acetate. In conclusion, a highfiber diet or one of its metabolic products, acetate, controls the inflammatory response to MSU crystals by favoring the resolution of the inflammatory response. Our studies suggest that what we eat plays a determinant role in our capacity to fine tune the inflammatory response.

Original languageEnglish
Pages (from-to)275-284
Number of pages10
JournalJournal of leukocyte biology
Volume101
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Arthritis
  • Immunoregulation
  • Microbiota

Cite this

Vieira, A. T., Galvão, I., Macia, L. M., Sernaglia, E. M., Vinolo, M. A. R., Garcia, C. C., ... Teixeira, M. M. (2017). Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice. Journal of leukocyte biology, 101(1), 275-284. https://doi.org/10.1189/jlb.3A1015-453RRR
Vieira, Angélica T. ; Galvão, Izabela ; Macia, Laurence M. ; Sernaglia, Erica M. ; Vinolo, Marco Aurélio R. ; Garcia, Cristiana Couto ; Tavares, Luciana Padua ; Amaral, Flávio A. ; Sousa, Lirlândia P. ; Martins, Flaviano S ; Mackay, Charles R. ; Teixeira, Mauro Martin. / Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice. In: Journal of leukocyte biology. 2017 ; Vol. 101, No. 1. pp. 275-284.
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title = "Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice",
abstract = "Gout is a disease characterized by the deposition of monosodium urate (MSU) crystals in the joints. Continuous gout episodes may lead to unresolved inflammatory responses and tissue damage. We investigated the effects of a high-fiber diet and acetate, a short-chain fatty acid (SCFA) resulting from the metabolism of fiber by gut microbiota, on the inflammatory response in an experimental model of gout in mice. Injection of MSU crystals into the knee joint of mice induced neutrophil influx and inflammatory hypernociception. The onset of inflammatory response induced by MSU crystals was not altered in animals given a high-fiber diet, but the highfiber diet induced faster resolution of the inflammatory response. Similar results were obtained in animals given the SCFA acetate. Acetate was effective, even when given after injection of MSU crystals at the peak of the inflammatory response and induced caspasedependent apoptosis of neutrophils that accounted for the resolution of inflammation. Resolution of neutrophilic inflammation was associated with decreased NF-κB activity and enhanced production of anti-inflammatory mediators, including IL-10, TGF-β, and annexin A1. Acetate treatment or intake of a high-fiber diet enhanced efferocytosis, an effect also observed in vitro with neutrophils treated with acetate. In conclusion, a highfiber diet or one of its metabolic products, acetate, controls the inflammatory response to MSU crystals by favoring the resolution of the inflammatory response. Our studies suggest that what we eat plays a determinant role in our capacity to fine tune the inflammatory response.",
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Vieira, AT, Galvão, I, Macia, LM, Sernaglia, EM, Vinolo, MAR, Garcia, CC, Tavares, LP, Amaral, FA, Sousa, LP, Martins, FS, Mackay, CR & Teixeira, MM 2017, 'Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice', Journal of leukocyte biology, vol. 101, no. 1, pp. 275-284. https://doi.org/10.1189/jlb.3A1015-453RRR

Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice. / Vieira, Angélica T.; Galvão, Izabela; Macia, Laurence M.; Sernaglia, Erica M.; Vinolo, Marco Aurélio R.; Garcia, Cristiana Couto; Tavares, Luciana Padua; Amaral, Flávio A.; Sousa, Lirlândia P.; Martins, Flaviano S; Mackay, Charles R.; Teixeira, Mauro Martin.

In: Journal of leukocyte biology, Vol. 101, No. 1, 01.01.2017, p. 275-284.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice

AU - Vieira, Angélica T.

AU - Galvão, Izabela

AU - Macia, Laurence M.

AU - Sernaglia, Erica M.

AU - Vinolo, Marco Aurélio R.

AU - Garcia, Cristiana Couto

AU - Tavares, Luciana Padua

AU - Amaral, Flávio A.

AU - Sousa, Lirlândia P.

AU - Martins, Flaviano S

AU - Mackay, Charles R.

AU - Teixeira, Mauro Martin

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Gout is a disease characterized by the deposition of monosodium urate (MSU) crystals in the joints. Continuous gout episodes may lead to unresolved inflammatory responses and tissue damage. We investigated the effects of a high-fiber diet and acetate, a short-chain fatty acid (SCFA) resulting from the metabolism of fiber by gut microbiota, on the inflammatory response in an experimental model of gout in mice. Injection of MSU crystals into the knee joint of mice induced neutrophil influx and inflammatory hypernociception. The onset of inflammatory response induced by MSU crystals was not altered in animals given a high-fiber diet, but the highfiber diet induced faster resolution of the inflammatory response. Similar results were obtained in animals given the SCFA acetate. Acetate was effective, even when given after injection of MSU crystals at the peak of the inflammatory response and induced caspasedependent apoptosis of neutrophils that accounted for the resolution of inflammation. Resolution of neutrophilic inflammation was associated with decreased NF-κB activity and enhanced production of anti-inflammatory mediators, including IL-10, TGF-β, and annexin A1. Acetate treatment or intake of a high-fiber diet enhanced efferocytosis, an effect also observed in vitro with neutrophils treated with acetate. In conclusion, a highfiber diet or one of its metabolic products, acetate, controls the inflammatory response to MSU crystals by favoring the resolution of the inflammatory response. Our studies suggest that what we eat plays a determinant role in our capacity to fine tune the inflammatory response.

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KW - Arthritis

KW - Immunoregulation

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