Abstract
Gout is a disease characterized by the deposition of monosodium urate (MSU) crystals in the joints. Continuous gout episodes may lead to unresolved inflammatory responses and tissue damage. We investigated the effects of a high-fiber diet and acetate, a short-chain fatty acid (SCFA) resulting from the metabolism of fiber by gut microbiota, on the inflammatory response in an experimental model of gout in mice. Injection of MSU crystals into the knee joint of mice induced neutrophil influx and inflammatory hypernociception. The onset of inflammatory response induced by MSU crystals was not altered in animals given a high-fiber diet, but the highfiber diet induced faster resolution of the inflammatory response. Similar results were obtained in animals given the SCFA acetate. Acetate was effective, even when given after injection of MSU crystals at the peak of the inflammatory response and induced caspasedependent apoptosis of neutrophils that accounted for the resolution of inflammation. Resolution of neutrophilic inflammation was associated with decreased NF-κB activity and enhanced production of anti-inflammatory mediators, including IL-10, TGF-β, and annexin A1. Acetate treatment or intake of a high-fiber diet enhanced efferocytosis, an effect also observed in vitro with neutrophils treated with acetate. In conclusion, a highfiber diet or one of its metabolic products, acetate, controls the inflammatory response to MSU crystals by favoring the resolution of the inflammatory response. Our studies suggest that what we eat plays a determinant role in our capacity to fine tune the inflammatory response.
| Original language | English |
|---|---|
| Pages (from-to) | 275-284 |
| Number of pages | 10 |
| Journal | Journal of leukocyte biology |
| Volume | 101 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2017 |
Keywords
- Arthritis
- Immunoregulation
- Microbiota
Cite this
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Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice. / Vieira, Angélica T.; Galvão, Izabela; Macia, Laurence M.; Sernaglia, Erica M.; Vinolo, Marco Aurélio R.; Garcia, Cristiana Couto; Tavares, Luciana Padua; Amaral, Flávio A.; Sousa, Lirlândia P.; Martins, Flaviano S; Mackay, Charles R.; Teixeira, Mauro Martin.
In: Journal of leukocyte biology, Vol. 101, No. 1, 01.01.2017, p. 275-284.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice
AU - Vieira, Angélica T.
AU - Galvão, Izabela
AU - Macia, Laurence M.
AU - Sernaglia, Erica M.
AU - Vinolo, Marco Aurélio R.
AU - Garcia, Cristiana Couto
AU - Tavares, Luciana Padua
AU - Amaral, Flávio A.
AU - Sousa, Lirlândia P.
AU - Martins, Flaviano S
AU - Mackay, Charles R.
AU - Teixeira, Mauro Martin
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Gout is a disease characterized by the deposition of monosodium urate (MSU) crystals in the joints. Continuous gout episodes may lead to unresolved inflammatory responses and tissue damage. We investigated the effects of a high-fiber diet and acetate, a short-chain fatty acid (SCFA) resulting from the metabolism of fiber by gut microbiota, on the inflammatory response in an experimental model of gout in mice. Injection of MSU crystals into the knee joint of mice induced neutrophil influx and inflammatory hypernociception. The onset of inflammatory response induced by MSU crystals was not altered in animals given a high-fiber diet, but the highfiber diet induced faster resolution of the inflammatory response. Similar results were obtained in animals given the SCFA acetate. Acetate was effective, even when given after injection of MSU crystals at the peak of the inflammatory response and induced caspasedependent apoptosis of neutrophils that accounted for the resolution of inflammation. Resolution of neutrophilic inflammation was associated with decreased NF-κB activity and enhanced production of anti-inflammatory mediators, including IL-10, TGF-β, and annexin A1. Acetate treatment or intake of a high-fiber diet enhanced efferocytosis, an effect also observed in vitro with neutrophils treated with acetate. In conclusion, a highfiber diet or one of its metabolic products, acetate, controls the inflammatory response to MSU crystals by favoring the resolution of the inflammatory response. Our studies suggest that what we eat plays a determinant role in our capacity to fine tune the inflammatory response.
AB - Gout is a disease characterized by the deposition of monosodium urate (MSU) crystals in the joints. Continuous gout episodes may lead to unresolved inflammatory responses and tissue damage. We investigated the effects of a high-fiber diet and acetate, a short-chain fatty acid (SCFA) resulting from the metabolism of fiber by gut microbiota, on the inflammatory response in an experimental model of gout in mice. Injection of MSU crystals into the knee joint of mice induced neutrophil influx and inflammatory hypernociception. The onset of inflammatory response induced by MSU crystals was not altered in animals given a high-fiber diet, but the highfiber diet induced faster resolution of the inflammatory response. Similar results were obtained in animals given the SCFA acetate. Acetate was effective, even when given after injection of MSU crystals at the peak of the inflammatory response and induced caspasedependent apoptosis of neutrophils that accounted for the resolution of inflammation. Resolution of neutrophilic inflammation was associated with decreased NF-κB activity and enhanced production of anti-inflammatory mediators, including IL-10, TGF-β, and annexin A1. Acetate treatment or intake of a high-fiber diet enhanced efferocytosis, an effect also observed in vitro with neutrophils treated with acetate. In conclusion, a highfiber diet or one of its metabolic products, acetate, controls the inflammatory response to MSU crystals by favoring the resolution of the inflammatory response. Our studies suggest that what we eat plays a determinant role in our capacity to fine tune the inflammatory response.
KW - Arthritis
KW - Immunoregulation
KW - Microbiota
UR - http://www.scopus.com/inward/record.url?scp=85008429689&partnerID=8YFLogxK
U2 - 10.1189/jlb.3A1015-453RRR
DO - 10.1189/jlb.3A1015-453RRR
M3 - Article
VL - 101
SP - 275
EP - 284
JO - Journal of leukocyte biology
JF - Journal of leukocyte biology
SN - 0741-5400
IS - 1
ER -