Diet-derived short chain fatty acids stimulate intestinal epithelial cells to induce mucosal tolerogenic dendritic cells

Gera Goverse, Rosalie Molenaar, Laurence Macia, Jian Tan, Martje N. Erkelens, Tanja Konijn, Marlene Knippenberg, Emma C.L. Cook, Diana Hanekamp, Marc Veldhoen, Anita Hartog, Guus Roeselers, Charles R. MacKay, Reina E. Mebius

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Abstract

The gastrointestinal tract is continuously exposed to many environmental factors that influence intestinal epithelial cells and the underlying mucosal immune system. In this article, we demonstrate that dietary fiber and short chain fatty acids (SCFAs) induced the expression of the Vitamin A-converting enzyme RALDH1 in intestinal epithelial cells in vivo and in vitro, respectively. Furthermore, our data showed that the expression levels of RALDH1 in small intestinal epithelial cells correlated with the activity of Vitamin A-converting enzymes in mesenteric lymph node dendritic cells, along with increased numbers of intestinal regulatory T cells and a higher production of luminal IgA. Moreover, we show that the consumption of dietary fiber can alter the composition of SCFA-producing microbiota and SCFA production in the small intestines. In conclusion, our data illustrate that dietary adjustments affect small intestinal epithelial cells and can be used to modulate the mucosal immune system.

Original languageEnglish
Pages (from-to)2172-2181
Number of pages10
JournalJournal of Immunology
Volume198
Issue number5
DOIs
Publication statusPublished - 1 Mar 2017

Cite this

Goverse, G., Molenaar, R., Macia, L., Tan, J., Erkelens, M. N., Konijn, T., ... Mebius, R. E. (2017). Diet-derived short chain fatty acids stimulate intestinal epithelial cells to induce mucosal tolerogenic dendritic cells. Journal of Immunology, 198(5), 2172-2181. https://doi.org/10.4049/jimmunol.1600165
Goverse, Gera ; Molenaar, Rosalie ; Macia, Laurence ; Tan, Jian ; Erkelens, Martje N. ; Konijn, Tanja ; Knippenberg, Marlene ; Cook, Emma C.L. ; Hanekamp, Diana ; Veldhoen, Marc ; Hartog, Anita ; Roeselers, Guus ; MacKay, Charles R. ; Mebius, Reina E. / Diet-derived short chain fatty acids stimulate intestinal epithelial cells to induce mucosal tolerogenic dendritic cells. In: Journal of Immunology. 2017 ; Vol. 198, No. 5. pp. 2172-2181.
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abstract = "The gastrointestinal tract is continuously exposed to many environmental factors that influence intestinal epithelial cells and the underlying mucosal immune system. In this article, we demonstrate that dietary fiber and short chain fatty acids (SCFAs) induced the expression of the Vitamin A-converting enzyme RALDH1 in intestinal epithelial cells in vivo and in vitro, respectively. Furthermore, our data showed that the expression levels of RALDH1 in small intestinal epithelial cells correlated with the activity of Vitamin A-converting enzymes in mesenteric lymph node dendritic cells, along with increased numbers of intestinal regulatory T cells and a higher production of luminal IgA. Moreover, we show that the consumption of dietary fiber can alter the composition of SCFA-producing microbiota and SCFA production in the small intestines. In conclusion, our data illustrate that dietary adjustments affect small intestinal epithelial cells and can be used to modulate the mucosal immune system.",
author = "Gera Goverse and Rosalie Molenaar and Laurence Macia and Jian Tan and Erkelens, {Martje N.} and Tanja Konijn and Marlene Knippenberg and Cook, {Emma C.L.} and Diana Hanekamp and Marc Veldhoen and Anita Hartog and Guus Roeselers and MacKay, {Charles R.} and Mebius, {Reina E.}",
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Goverse, G, Molenaar, R, Macia, L, Tan, J, Erkelens, MN, Konijn, T, Knippenberg, M, Cook, ECL, Hanekamp, D, Veldhoen, M, Hartog, A, Roeselers, G, MacKay, CR & Mebius, RE 2017, 'Diet-derived short chain fatty acids stimulate intestinal epithelial cells to induce mucosal tolerogenic dendritic cells', Journal of Immunology, vol. 198, no. 5, pp. 2172-2181. https://doi.org/10.4049/jimmunol.1600165

Diet-derived short chain fatty acids stimulate intestinal epithelial cells to induce mucosal tolerogenic dendritic cells. / Goverse, Gera; Molenaar, Rosalie; Macia, Laurence; Tan, Jian; Erkelens, Martje N.; Konijn, Tanja; Knippenberg, Marlene; Cook, Emma C.L.; Hanekamp, Diana; Veldhoen, Marc; Hartog, Anita; Roeselers, Guus; MacKay, Charles R.; Mebius, Reina E.

In: Journal of Immunology, Vol. 198, No. 5, 01.03.2017, p. 2172-2181.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Diet-derived short chain fatty acids stimulate intestinal epithelial cells to induce mucosal tolerogenic dendritic cells

AU - Goverse, Gera

AU - Molenaar, Rosalie

AU - Macia, Laurence

AU - Tan, Jian

AU - Erkelens, Martje N.

AU - Konijn, Tanja

AU - Knippenberg, Marlene

AU - Cook, Emma C.L.

AU - Hanekamp, Diana

AU - Veldhoen, Marc

AU - Hartog, Anita

AU - Roeselers, Guus

AU - MacKay, Charles R.

AU - Mebius, Reina E.

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AB - The gastrointestinal tract is continuously exposed to many environmental factors that influence intestinal epithelial cells and the underlying mucosal immune system. In this article, we demonstrate that dietary fiber and short chain fatty acids (SCFAs) induced the expression of the Vitamin A-converting enzyme RALDH1 in intestinal epithelial cells in vivo and in vitro, respectively. Furthermore, our data showed that the expression levels of RALDH1 in small intestinal epithelial cells correlated with the activity of Vitamin A-converting enzymes in mesenteric lymph node dendritic cells, along with increased numbers of intestinal regulatory T cells and a higher production of luminal IgA. Moreover, we show that the consumption of dietary fiber can alter the composition of SCFA-producing microbiota and SCFA production in the small intestines. In conclusion, our data illustrate that dietary adjustments affect small intestinal epithelial cells and can be used to modulate the mucosal immune system.

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