Dielectrophoretic-activated cell sorter based on curved microelectrodes

Khashayar Khoshmanesh, Chen Zhang, Francisco Javier Tovar-Lopez, Saeid Nahavandi, Sara Baratchi, Arnan Mitchell, Kourosh Kalantar-Zadeh

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51 Citations (Scopus)

Abstract

This article presents the numerical and experimental analysis of a dielectrophoretic-activated cell sorter (DACS), which is equipped with curved microelectrodes. Curved microelectrodes offer unique advantages, since they create strong dielectrophoretic (DEP) forces over the tips and maintain it over a large portion of their structure, as predicted by simulations. The performance of the system is assessed using yeast (Saccharomyces cerevisiae) cells as model organisms. The separation of the live and dead cells is demonstrated at different medium conductivities of 0.001 and 0.14 S/m, and the sorting performance was assessed using a second array of microelectrodes patterned downstream the microchannel. Further, microscopic cell counting analysis reveals that a single pass through the system yields a separating efficiency of *80 at low medium conductivities and *85 at high medium conductivities.
Original languageEnglish
Pages (from-to)411 - 426
Number of pages16
JournalMicrofluidics and Nanofluidics
Volume9
Issue number2-3
DOIs
Publication statusPublished - 2010
Externally publishedYes

Cite this

Khoshmanesh, K., Zhang, C., Tovar-Lopez, F. J., Nahavandi, S., Baratchi, S., Mitchell, A., & Kalantar-Zadeh, K. (2010). Dielectrophoretic-activated cell sorter based on curved microelectrodes. Microfluidics and Nanofluidics, 9(2-3), 411 - 426. https://doi.org/10.1007/s10404-009-0558-7