TY - JOUR
T1 - Didemnin B induces cell death by apoptosis
T2 - The fastest induction of apoptosis ever described
AU - Grubb, David R.
AU - Wolvetang, Ernst J.
AU - Lawen, Alfons
PY - 1995/10/24
Y1 - 1995/10/24
N2 - Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 μM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within the cytosol and the generation of DNA ladders. None of these characteristics of apoptosis are seen when HL-60 cells are pretreated with 1 mM Zn2+ immediately before treatment with didemnin B.
AB - Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 μM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within the cytosol and the generation of DNA ladders. None of these characteristics of apoptosis are seen when HL-60 cells are pretreated with 1 mM Zn2+ immediately before treatment with didemnin B.
UR - http://www.scopus.com/inward/record.url?scp=0028815543&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1995.2580
DO - 10.1006/bbrc.1995.2580
M3 - Article
AN - SCOPUS:0028815543
VL - 215
SP - 1130
EP - 1136
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -