Didemnin B induces cell death by apoptosis: The fastest induction of apoptosis ever described

David R. Grubb, Ernst J. Wolvetang, Alfons Lawen

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Abstract

Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 μM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within the cytosol and the generation of DNA ladders. None of these characteristics of apoptosis are seen when HL-60 cells are pretreated with 1 mM Zn2+ immediately before treatment with didemnin B.

Original languageEnglish
Pages (from-to)1130-1136
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume215
Issue number3
DOIs
Publication statusPublished - 24 Oct 1995

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