Diagnosis of Endometriosis

Proteomics

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

Abstract

Proteomics techniques have been applied to identify proteins for development as diagnostic markers of endometriosis, but to date most studies have been unable to overcome the presence of highly abundant proteins. The main issues to be resolved include the choice of sample for discovery of new markers (eutopic or ectopic tissue, peritoneal fluid, uterine lavage fluid or blood) and the classification of test samples. The application of emerging fractionation techniques to capture and concentrate low-abundance proteins, new non-gel-based proteomic technologies and various protein labeling strategies combined, and the ongoing development of advanced bio-informatics tools should enable progress towards the development of relatively non-invasive diagnostic methods for this disease. 

Original languageEnglish
Title of host publicationEndometriosis
Subtitle of host publicationScience and Practice
EditorsLinda C. Giudice, Johannes L.H. Evers, David L. Healy
Place of PublicationWest Essex UK
PublisherWiley-Blackwell
Pages324-335
Number of pages12
ISBN (Print)9781444332131
DOIs
Publication statusPublished - 25 Jan 2012

Keywords

  • Diagnostic markers
  • DIGE
  • Endometrial proteome
  • Endometriosis
  • Proteomic techniques
  • Proteomics
  • Sampling
  • SELDI-TOF-MS
  • Uterine

Cite this

Stephens, A. N., Rombauts, L. J. F., & Salamonsen, L. A. (2012). Diagnosis of Endometriosis: Proteomics. In L. C. Giudice, J. L. H. Evers, & D. L. Healy (Eds.), Endometriosis: Science and Practice (pp. 324-335). West Essex UK: Wiley-Blackwell. https://doi.org/10.1002/9781444398519.ch31
Stephens, Andrew N. ; Rombauts, Luk J. F. ; Salamonsen, Lois A. / Diagnosis of Endometriosis : Proteomics. Endometriosis: Science and Practice. editor / Linda C. Giudice ; Johannes L.H. Evers ; David L. Healy. West Essex UK : Wiley-Blackwell, 2012. pp. 324-335
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Stephens, AN, Rombauts, LJF & Salamonsen, LA 2012, Diagnosis of Endometriosis: Proteomics. in LC Giudice, JLH Evers & DL Healy (eds), Endometriosis: Science and Practice. Wiley-Blackwell, West Essex UK, pp. 324-335. https://doi.org/10.1002/9781444398519.ch31

Diagnosis of Endometriosis : Proteomics. / Stephens, Andrew N.; Rombauts, Luk J. F.; Salamonsen, Lois A.

Endometriosis: Science and Practice. ed. / Linda C. Giudice; Johannes L.H. Evers; David L. Healy. West Essex UK : Wiley-Blackwell, 2012. p. 324-335.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

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N2 - Proteomics techniques have been applied to identify proteins for development as diagnostic markers of endometriosis, but to date most studies have been unable to overcome the presence of highly abundant proteins. The main issues to be resolved include the choice of sample for discovery of new markers (eutopic or ectopic tissue, peritoneal fluid, uterine lavage fluid or blood) and the classification of test samples. The application of emerging fractionation techniques to capture and concentrate low-abundance proteins, new non-gel-based proteomic technologies and various protein labeling strategies combined, and the ongoing development of advanced bio-informatics tools should enable progress towards the development of relatively non-invasive diagnostic methods for this disease. 

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KW - Proteomic techniques

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KW - Sampling

KW - SELDI-TOF-MS

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Stephens AN, Rombauts LJF, Salamonsen LA. Diagnosis of Endometriosis: Proteomics. In Giudice LC, Evers JLH, Healy DL, editors, Endometriosis: Science and Practice. West Essex UK: Wiley-Blackwell. 2012. p. 324-335 https://doi.org/10.1002/9781444398519.ch31