Developmental fate determination and marker discovery in hematopoietic stem cell biology using proteomic fingerprinting

Elaine Spooncer, Natalie Brouard, Susie K Nilsson, Brenda Williams, Mira C Liu, Richard D Unwin, David Blinco, Ewa Jaworska, Paul J Simmons, Anthony D Whetton

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

Abstract

In hematopoiesis, co-expression of Sca-1 and c-Kit defines cells (LS(+)K) with long term reconstituting potential. In contrast, poorly characterized LS(-)K cells fail to reconstitute lethally irradiated recipients. Relative quantification mass spectrometry and transcriptional profiling were used to characterize LS(+)K and LS(-)K cells. This approach yielded data on >1200 proteins. Only 32 of protein changes correlated to mRNA modulation demonstrating post-translational protein regulation in early hematopoietic development. LS(+)K cells had lower expression of protein synthesis proteins but did express proteins associated with mature cell function. Major increases in erythroid development proteins were observed in LS(-)K cells; based on this assessment of erythroid potential we showed them to be principally erythroid progenitors, demonstrating effective use of discovery proteomics for definition of primitive cells.
Original languageEnglish
Pages (from-to)573 - 581
Number of pages9
JournalMolecular & Cellular Proteomics
Volume7
Issue number3
DOIs
Publication statusPublished - 2008

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