Development of synchrotron radiation x-ray intravital microscopy for in vivo imaging of rat heart vascular function

Keiji Umetani; James Todd Pearson; Daryl Schwenke; Mikiyasu Shirai

doi:10.1109/IEMBS.2011.6091920

Development of synchrotron radiation x-ray intravital microscopy for in vivo imaging of rat heart vascular function

Keiji Umetani, James Todd Pearson, Daryl Schwenke, Mikiyasu Shirai

Physiology

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

8 Citations (Scopus)

Abstract

This study elucidates the vascular internal diameter response of coronary arterial circulation in closed-chest rats to evaluate endothelium-dependent and endothelium-independent vasodilatory ability and to investigate disease mechanisms. For this study, we developed an X-ray intravital microscopy system using a microangiography technique and a synchrotron radiation source at SPring-8. An X-ray direct-conversion type detector with 7-mum spatial resolution was used for real-time imaging. Microangiographic images were stored in a digital frame memory system at a maximum rate of 30 frame/s with a 1024 x 1024-pixel, 10-bit format. In imaging experiments, the small coronary arteries were visualized after iodine contrast agent injection into the coronary artery.

Original language	English
Title of host publication	33rd Annual International Conference of the IEEE EMBS
Editors	N H Lovell
Place of Publication	USA
Publisher	IEEE, Institute of Electrical and Electronics Engineers
Pages	7791 - 7794
Number of pages	4
ISBN (Print)	9781424441228
DOIs	https://doi.org/10.1109/IEMBS.2011.6091920
Publication status	Published - 2011
Event	International Conference of the IEEE Engineering in Medicine and Biology Society 2011 - Boston, United States of America Duration: 30 Aug 2011 → 3 Sept 2011 Conference number: 33rd https://ieeexplore.ieee.org/xpl/conhome/6067544/proceeding (Proceedings)

Conference

Conference	International Conference of the IEEE Engineering in Medicine and Biology Society 2011
Abbreviated title	EMBC 2011
Country/Territory	United States of America
City	Boston
Period	30/08/11 → 3/09/11
Internet address	https://ieeexplore.ieee.org/xpl/conhome/6067544/proceeding (Proceedings)

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10.1109/IEMBS.2011.6091920

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Umetani, K., Pearson, J. T., Schwenke, D., & Shirai, M. (2011). Development of synchrotron radiation x-ray intravital microscopy for in vivo imaging of rat heart vascular function. In N. H. Lovell (Ed.), 33rd Annual International Conference of the IEEE EMBS (pp. 7791 - 7794). IEEE, Institute of Electrical and Electronics Engineers. https://doi.org/10.1109/IEMBS.2011.6091920

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title = "Development of synchrotron radiation x-ray intravital microscopy for in vivo imaging of rat heart vascular function",

abstract = "This study elucidates the vascular internal diameter response of coronary arterial circulation in closed-chest rats to evaluate endothelium-dependent and endothelium-independent vasodilatory ability and to investigate disease mechanisms. For this study, we developed an X-ray intravital microscopy system using a microangiography technique and a synchrotron radiation source at SPring-8. An X-ray direct-conversion type detector with 7-mum spatial resolution was used for real-time imaging. Microangiographic images were stored in a digital frame memory system at a maximum rate of 30 frame/s with a 1024 x 1024-pixel, 10-bit format. In imaging experiments, the small coronary arteries were visualized after iodine contrast agent injection into the coronary artery.",

author = "Keiji Umetani and Pearson, \{James Todd\} and Daryl Schwenke and Mikiyasu Shirai",

year = "2011",

doi = "10.1109/IEMBS.2011.6091920",

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isbn = "9781424441228",

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booktitle = "33rd Annual International Conference of the IEEE EMBS",

publisher = "IEEE, Institute of Electrical and Electronics Engineers",

address = "United States of America",

note = "International Conference of the IEEE Engineering in Medicine and Biology Society 2011, EMBC 2011 ; Conference date: 30-08-2011 Through 03-09-2011",

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Umetani, K, Pearson, JT, Schwenke, D & Shirai, M 2011, Development of synchrotron radiation x-ray intravital microscopy for in vivo imaging of rat heart vascular function. in NH Lovell (ed.), 33rd Annual International Conference of the IEEE EMBS. IEEE, Institute of Electrical and Electronics Engineers, USA, pp. 7791 - 7794, International Conference of the IEEE Engineering in Medicine and Biology Society 2011, Boston, Massachusetts, United States of America, 30/08/11. https://doi.org/10.1109/IEMBS.2011.6091920

Development of synchrotron radiation x-ray intravital microscopy for in vivo imaging of rat heart vascular function. / Umetani, Keiji; Pearson, James Todd; Schwenke, Daryl et al.
33rd Annual International Conference of the IEEE EMBS. ed. / N H Lovell. USA: IEEE, Institute of Electrical and Electronics Engineers, 2011. p. 7791 - 7794.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

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N2 - This study elucidates the vascular internal diameter response of coronary arterial circulation in closed-chest rats to evaluate endothelium-dependent and endothelium-independent vasodilatory ability and to investigate disease mechanisms. For this study, we developed an X-ray intravital microscopy system using a microangiography technique and a synchrotron radiation source at SPring-8. An X-ray direct-conversion type detector with 7-mum spatial resolution was used for real-time imaging. Microangiographic images were stored in a digital frame memory system at a maximum rate of 30 frame/s with a 1024 x 1024-pixel, 10-bit format. In imaging experiments, the small coronary arteries were visualized after iodine contrast agent injection into the coronary artery.

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Development of synchrotron radiation x-ray intravital microscopy for in vivo imaging of rat heart vascular function

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