Development of synchrotron radiation x-ray intravital microscopy for in vivo imaging of rat heart vascular function

Keiji Umetani, James Todd Pearson, Daryl Schwenke, Mikiyasu Shirai

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

6 Citations (Scopus)


This study elucidates the vascular internal diameter response of coronary arterial circulation in closed-chest rats to evaluate endothelium-dependent and endothelium-independent vasodilatory ability and to investigate disease mechanisms. For this study, we developed an X-ray intravital microscopy system using a microangiography technique and a synchrotron radiation source at SPring-8. An X-ray direct-conversion type detector with 7-mum spatial resolution was used for real-time imaging. Microangiographic images were stored in a digital frame memory system at a maximum rate of 30 frame/s with a 1024 x 1024-pixel, 10-bit format. In imaging experiments, the small coronary arteries were visualized after iodine contrast agent injection into the coronary artery.
Original languageEnglish
Title of host publication33rd Annual International Conference of the IEEE EMBS
EditorsN H Lovell
Place of PublicationUSA
PublisherIEEE, Institute of Electrical and Electronics Engineers
Pages7791 - 7794
Number of pages4
ISBN (Print)1557-170X
Publication statusPublished - 2011
EventInternational Conference of the IEEE Engineering in Medicine and Biology Society 2011 - Boston Marriott Copley Place Hotel, Boston, United States of America
Duration: 30 Aug 20113 Sep 2011
Conference number: 33rd


ConferenceInternational Conference of the IEEE Engineering in Medicine and Biology Society 2011
Abbreviated titleEMBC 2011
CountryUnited States of America

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