TY - JOUR
T1 - Development of novel amperometric urea biosensor based on Fc-PAMAM and MWCNT bio-nanocomposite film
AU - Dervisevic, Esma
AU - Dervisevic, Muamer
AU - Nyangwebah, Jackson Narkpa
AU - Şenel, Mehmet
PY - 2017/7
Y1 - 2017/7
N2 - In this work, fabrication of new electrochemical urea biosensor based on ferrocene-poly(amidoamine) (Fc-PAMAM) dendrimers incorporated with multi walled carbon nanotubes (MWCNTs) is reported. Bio-sensing electrode was characterized using cyclic voltammogram where optimization studies were performed for reusability, storage, interference behaviors together with optimum temperature and pH parameters. Electrode showed excellent performance in urea detection with wide linear range of 0.2–1.8 mM, detection limit of 0.05 mM, fast response of 3 s, and sensitivity of 1.085 μA/cm2/mM. Final assessment of electrochemical biosensor was real sample application which was performed in healthy human blood sample where excellent performance in selectivity and sensitivity of the bio-electrode was observed. Fabricated bio-sensing electrode has great potential in urea measurement applications.
AB - In this work, fabrication of new electrochemical urea biosensor based on ferrocene-poly(amidoamine) (Fc-PAMAM) dendrimers incorporated with multi walled carbon nanotubes (MWCNTs) is reported. Bio-sensing electrode was characterized using cyclic voltammogram where optimization studies were performed for reusability, storage, interference behaviors together with optimum temperature and pH parameters. Electrode showed excellent performance in urea detection with wide linear range of 0.2–1.8 mM, detection limit of 0.05 mM, fast response of 3 s, and sensitivity of 1.085 μA/cm2/mM. Final assessment of electrochemical biosensor was real sample application which was performed in healthy human blood sample where excellent performance in selectivity and sensitivity of the bio-electrode was observed. Fabricated bio-sensing electrode has great potential in urea measurement applications.
KW - Amperometric biosensor
KW - Bionanocomposite
KW - Ferrocence
KW - MWCNT
KW - PAMAM dendrimer
KW - Urea
KW - Urease
UR - http://www.scopus.com/inward/record.url?scp=85014178276&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2017.02.122
DO - 10.1016/j.snb.2017.02.122
M3 - Article
AN - SCOPUS:85014178276
SN - 0925-4005
VL - 246
SP - 920
EP - 926
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -
