Development of glucose biosensor based on reconstitution of glucose oxidase onto polymeric redox mediator coated pencil graphite electrodes

Muamer Dervisevic, Emre Çevik, Mehmet Şenel

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Abstract

In this study, a novel glucose biosensor was abricated by reconstitutional immobilization of glucose oxidase (GOx) onto a poly(glycidyl methacrylate-co-vinylferrocene) (poly(GMA-co-VFc)) film coated pencil graphite electrode (PGE). The amperometric current response of poly(GMA-co-VFc)-GOx to glucose is linear in the concentration range between 1 and 16. mM (correlation coefficient of 0.9998) with a detection limit of 2.7. μM (S/. N = 3). Experimental parameters were studied in detail and optimized, including the pH and temperature governing the analytical performance of the biosensor. The stability and reusability of the biosensor as well as its kinetic parameters have also been studied.

Original languageEnglish
Pages (from-to)69-76
Number of pages8
JournalEnzyme and Microbial Technology
Volume68
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Keywords

  • Amperometric biosensor
  • Ferrocene
  • Glucose
  • Mediator

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