Development of chorion-less zebrafish embryos in millifluidic living embryo arrays

Nurul Mohd Fuad, Jan Kaslin, Donald Wlodkowic

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.

Original languageEnglish
Article number051101-1
Number of pages5
JournalBiomicrofluidics
Volume11
Issue number5
DOIs
Publication statusPublished - 1 Sep 2017

Cite this

@article{0eeedb83ebcb49f6a74acaa75274efa5,
title = "Development of chorion-less zebrafish embryos in millifluidic living embryo arrays",
abstract = "Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.",
author = "Fuad, {Nurul Mohd} and Jan Kaslin and Donald Wlodkowic",
year = "2017",
month = "9",
day = "1",
doi = "10.1063/1.5001848",
language = "English",
volume = "11",
journal = "Biomicrofluidics",
issn = "1932-1058",
publisher = "AIP Publishing",
number = "5",

}

Development of chorion-less zebrafish embryos in millifluidic living embryo arrays. / Fuad, Nurul Mohd; Kaslin, Jan; Wlodkowic, Donald.

In: Biomicrofluidics, Vol. 11, No. 5, 051101-1, 01.09.2017.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Development of chorion-less zebrafish embryos in millifluidic living embryo arrays

AU - Fuad, Nurul Mohd

AU - Kaslin, Jan

AU - Wlodkowic, Donald

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.

AB - Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.

UR - http://www.scopus.com/inward/record.url?scp=85030631947&partnerID=8YFLogxK

U2 - 10.1063/1.5001848

DO - 10.1063/1.5001848

M3 - Article

VL - 11

JO - Biomicrofluidics

JF - Biomicrofluidics

SN - 1932-1058

IS - 5

M1 - 051101-1

ER -