Abstract
Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.
| Original language | English |
|---|---|
| Article number | 051101-1 |
| Number of pages | 5 |
| Journal | Biomicrofluidics |
| Volume | 11 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 Sep 2017 |
Cite this
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Development of chorion-less zebrafish embryos in millifluidic living embryo arrays. / Fuad, Nurul Mohd; Kaslin, Jan; Wlodkowic, Donald.
In: Biomicrofluidics, Vol. 11, No. 5, 051101-1, 01.09.2017.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Development of chorion-less zebrafish embryos in millifluidic living embryo arrays
AU - Fuad, Nurul Mohd
AU - Kaslin, Jan
AU - Wlodkowic, Donald
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.
AB - Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.
UR - http://www.scopus.com/inward/record.url?scp=85030631947&partnerID=8YFLogxK
U2 - 10.1063/1.5001848
DO - 10.1063/1.5001848
M3 - Article
VL - 11
JO - Biomicrofluidics
JF - Biomicrofluidics
SN - 1932-1058
IS - 5
M1 - 051101-1
ER -