Abstract
Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.
Original language | English |
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Article number | 051101-1 |
Number of pages | 5 |
Journal | Biomicrofluidics |
Volume | 11 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1 Sep 2017 |