Development of chorion-less zebrafish embryos in millifluidic living embryo arrays

Nurul Mohd Fuad, Jan Kaslin, Donald Wlodkowic

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6 Citations (Scopus)

Abstract

Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.

Original languageEnglish
Article number051101-1
Number of pages5
JournalBiomicrofluidics
Volume11
Issue number5
DOIs
Publication statusPublished - 1 Sep 2017

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