Development of an Ultrasensitive Impedimetric Immunosensor Platform for Detection of Plasmodium Lactate Dehydrogenase

Yu Kong Low, Jianxiong Chan, Gita V. Soraya, Christelle Buffet, Chathurika D. Abeyrathne, Duc H. Huynh, Efstratios Skafidas, Patrick Kwan, Stephen J. Rogerson

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Elimination of malaria is a global health priority. Detecting an asymptomatic carrier of Plasmodium parasites to receive treatment is an important step in achieving this goal. Current available tools for detection of malaria parasites are either expensive, lacking in sensitivity for asymptomatic carriers, or low in throughput. We investigated the sensitivity of an impedimetric biosensor targeting the malaria biomarker Plasmodium lactate dehydrogenase (pLDH). Following optimization of the detection protocol, sensor performance was tested using phosphate-buffered saline (PBS), and then saliva samples spiked with pLDH at various concentrations. The presence of pLDH was determined by analyzing the sensor electrical properties before and after sample application. Through comparing percentage changes in impedance magnitude, the sensors distinguished pLDH-spiked PBS from non-spiked PBS at concentrations as low as 250 pg/mL (p = 0.0008). Percentage changes in impedance magnitude from saliva spiked with 2.5 ng/mL pLDH trended higher than those from non-spiked saliva. These results suggest that these biosensors have the potential to detect concentrations of pLDH up to two logs lower than currently available best-practice diagnostic tools. Successful optimization of this sensor platform would enable more efficient diagnosis of asymptomatic carriers, who can be targeted for treatment, contributing to the elimination of malaria.

Original languageEnglish
Article number2446
Number of pages10
JournalSensors
Volume19
Issue number11
DOIs
Publication statusPublished - 29 May 2019
Externally publishedYes

Keywords

  • biosensor
  • immunosensor
  • impedance
  • malaria
  • pLDH
  • ultrasensitive

Cite this

Low, Y. K., Chan, J., Soraya, G. V., Buffet, C., Abeyrathne, C. D., Huynh, D. H., ... Rogerson, S. J. (2019). Development of an Ultrasensitive Impedimetric Immunosensor Platform for Detection of Plasmodium Lactate Dehydrogenase. Sensors, 19(11), [2446]. https://doi.org/10.3390/s19112446
Low, Yu Kong ; Chan, Jianxiong ; Soraya, Gita V. ; Buffet, Christelle ; Abeyrathne, Chathurika D. ; Huynh, Duc H. ; Skafidas, Efstratios ; Kwan, Patrick ; Rogerson, Stephen J. / Development of an Ultrasensitive Impedimetric Immunosensor Platform for Detection of Plasmodium Lactate Dehydrogenase. In: Sensors. 2019 ; Vol. 19, No. 11.
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Low, YK, Chan, J, Soraya, GV, Buffet, C, Abeyrathne, CD, Huynh, DH, Skafidas, E, Kwan, P & Rogerson, SJ 2019, 'Development of an Ultrasensitive Impedimetric Immunosensor Platform for Detection of Plasmodium Lactate Dehydrogenase', Sensors, vol. 19, no. 11, 2446. https://doi.org/10.3390/s19112446

Development of an Ultrasensitive Impedimetric Immunosensor Platform for Detection of Plasmodium Lactate Dehydrogenase. / Low, Yu Kong; Chan, Jianxiong; Soraya, Gita V.; Buffet, Christelle; Abeyrathne, Chathurika D.; Huynh, Duc H.; Skafidas, Efstratios; Kwan, Patrick; Rogerson, Stephen J.

In: Sensors, Vol. 19, No. 11, 2446, 29.05.2019.

Research output: Contribution to journalArticleResearchpeer-review

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