@inproceedings{1ebfc9d3ce054989aa4799d76db8f208,
title = "Development of an electro-responsive platform for the controlled transfection of mammalian cells",
abstract = "The recent development of living microarrays as novel tools for the analysis of gene expression in an in-situ environment promises to unravel gene function within living organisms. In order to significantly enhance microarray performance, we are working towards electro-responsive DNA transfection chips. This study focuses on the control of DNA adsorption and desorption by appropriate surface modification of highly doped p++ silicon. Silicon was modified by plasma polymerisation of allylamine (ALAPP), a non-toxic surface that sustains cell growth. Subsequent high surface density grafting of poly(ethylene oxide) formed a layer resistant to biomolecule adsorption and cell attachment. Spatially controlled excimer laser ablation of the surface produced micron resolution patterns of re-exposed plasma polymer whilst the rest of the surface remained non-fouling. We observed electro-stimulated preferential adsorption of DNA to the ALAPP surface and subsequent desorption by the application of a negative bias. Cell culture experiments with HEK 293 cells demonstrated efficient and controlled transfection of cells using the expression of green fluorescent protein as a reporter. Thus, these chemically patterned surfaces are promising platforms for use as living microarrays.",
keywords = "Adsorption, Desorption, DNA, Non-fouling, PEO, Plasma polymerisation, Surface modification, Transfection",
author = "Hook, {Andrew L.} and Helmut Thissen and Hayes, {Jason P.} and Voelcker, {Nicolas H.}",
year = "2005",
month = jul,
day = "21",
doi = "10.1117/12.606789",
language = "English",
volume = "5651",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
pages = "418--426",
booktitle = "Proceedings",
note = "Biomedical Applications of Micro- and Nanoengineering II 2004 ; Conference date: 13-12-2004 Through 15-12-2004",
}