Development of Amperometric glucose biosensor based on reconstitution of glucose oxidase on polymeric 3-Aminophenyl Boronic Acid Monolayer

Mehmet Şenel, Cevdet Nergiz, Muamer Dervisevic, Emre Çevik

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23 Citations (Scopus)

Abstract

An amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and subsequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is described. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction and oxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at +0.5V with a high sensitivity (5.94μA/mM) and relatively good response time (∼12s) in a wide concentration range of 1-17mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.

Original languageEnglish
Pages (from-to)1194-1200
Number of pages7
JournalElectroanalysis
Volume25
Issue number5
DOIs
Publication statusPublished - May 2013
Externally publishedYes

Keywords

  • Biosensor
  • Boronic acid
  • Glucose Oxidase
  • Polyaniline

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