Development of Amperometric glucose biosensor based on reconstitution of glucose oxidase on polymeric 3-Aminophenyl Boronic Acid Monolayer

Mehmet Şenel; Cevdet Nergiz; Muamer Dervisevic; Emre Çevik

doi:10.1002/elan.201200535

Development of Amperometric glucose biosensor based on reconstitution of glucose oxidase on polymeric 3-Aminophenyl Boronic Acid Monolayer

Mehmet Şenel, Cevdet Nergiz, Muamer Dervisevic, Emre Çevik

Research output: Contribution to journal › Article › Research › peer-review

27 Citations (Scopus)

Abstract

An amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and subsequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is described. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction and oxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at +0.5V with a high sensitivity (5.94μA/mM) and relatively good response time (∼12s) in a wide concentration range of 1-17mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.

Original language	English
Pages (from-to)	1194-1200
Number of pages	7
Journal	Electroanalysis
Volume	25
Issue number	5
DOIs	https://doi.org/10.1002/elan.201200535
Publication status	Published - May 2013
Externally published	Yes

Keywords

Biosensor
Boronic acid
Glucose Oxidase
Polyaniline

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abstract = "An amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and subsequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is described. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction and oxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at +0.5V with a high sensitivity (5.94μA/mM) and relatively good response time (∼12s) in a wide concentration range of 1-17mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.",

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AU - Şenel, Mehmet

AU - Nergiz, Cevdet

AU - Dervisevic, Muamer

AU - Çevik, Emre

PY - 2013/5

Y1 - 2013/5

N2 - An amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and subsequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is described. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction and oxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at +0.5V with a high sensitivity (5.94μA/mM) and relatively good response time (∼12s) in a wide concentration range of 1-17mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.

AB - An amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and subsequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is described. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction and oxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at +0.5V with a high sensitivity (5.94μA/mM) and relatively good response time (∼12s) in a wide concentration range of 1-17mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.

KW - Biosensor

KW - Boronic acid

KW - Glucose Oxidase

KW - Polyaniline

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VL - 25

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JO - Electroanalysis

JF - Electroanalysis

IS - 5

ER -

Development of Amperometric glucose biosensor based on reconstitution of glucose oxidase on polymeric 3-Aminophenyl Boronic Acid Monolayer

Abstract

Keywords

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