Development of a synthetic consensus sequence scrambled antigen HIV-1 vaccine designed for global use

Scott A. Thomson, Angel B. Jaramillo, Maryanne Shoobridge, Kerrie J. Dunstan, Beth Everett, Charani Ranasinghe, Stephen J. Kent, Ke Gao, Jill Medveckzy, Rosemary A. Ffrench, Ian A. Ramshaw

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Abstract

Induction of high levels of broadly reactive cytotoxic T lymphocytes (CTL) remains a promising approach for an effective HIV-1 vaccine. We have developed a novel genetic-based vaccine strategy that encodes consensus overlapping peptide sets from all HIV-1 proteins scrambled together. This synthetic scrambled antigen vaccine (SAVINE) strategy has significant advantages, e.g. capacity to encode more antigens safely and is very flexible compared to traditional isolate-based strategies. The SAVINE vaccine strategy is clearly immunogenic, being able to restimulate a range of human HIV-1 specific responses in vitro and induce HIV-1 specific immunity in vivo in mice. Interestingly, different in vivo delivery strategies affected the resulting immunity and immunodominance pattern in mice. This platform strategy could be used for other infections and cancers where T cell responses are important for protection.

Original languageEnglish
Pages (from-to)4647-4657
Number of pages11
JournalVaccine
Volume23
Issue number38
DOIs
Publication statusPublished - 7 Sep 2005
Externally publishedYes

Keywords

  • Synthetic HIV vaccine

Cite this

Thomson, S. A., Jaramillo, A. B., Shoobridge, M., Dunstan, K. J., Everett, B., Ranasinghe, C., Kent, S. J., Gao, K., Medveckzy, J., Ffrench, R. A., & Ramshaw, I. A. (2005). Development of a synthetic consensus sequence scrambled antigen HIV-1 vaccine designed for global use. Vaccine, 23(38), 4647-4657. https://doi.org/10.1016/j.vaccine.2005.04.045