Development of a simple, sensitive, rapid test which discriminates BCG-vaccinated from Mycobacterium bovis-infected cattle

Paul Sopp, Michael Coad, Glyn R Hewinson, Chris John Howard, Cliona Ni Cheallaigh, Joseph Keane, James Harris, Jayne C Hope

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Bovine tuberculosis (bTB) is increasing in incidence in the UK. Effective control strategies could involve vaccination; BCG, either alone or in prime-boost strategies, remains the most effective vaccine against bovine tuberculosis. However, BCG vaccination of cattle would require development of diagnostic tests able to accurately discriminate Mycobacterium bovis-infected from BCG-vaccinated animals. Herein, we demonstrate that the detection of secreted IFN-gamma following short term culture (4h) of whole blood with purified protein derived from M. bovis (PPD-B) allows such discrimination. This reflects, in part, the differential kinetics of IFN-gamma secretion in infected compared to vaccinated cattle. This is the first study to demonstrate that accurate, rapid distinction of BCG-vaccinated from M. bovis-infected cattle can be achieved in a short time period without the need for production of M. bovis-specific antigens, complex antigen mixtures or extensive laboratory procedures. We were also able to detect PPD-specific IFN-gamma release during short term culture of blood from a number of humans with active TB indicating that this test may have wider application and is potentially useful for the rapid diagnosis of disease in humans.
Original languageEnglish
Pages (from-to)5470 - 5476
Number of pages7
JournalVaccine
Volume26
Issue number43
DOIs
Publication statusPublished - 2008

Cite this

Sopp, P., Coad, M., Hewinson, G. R., Howard, C. J., Cheallaigh, C. N., Keane, J., ... Hope, J. C. (2008). Development of a simple, sensitive, rapid test which discriminates BCG-vaccinated from Mycobacterium bovis-infected cattle. Vaccine, 26(43), 5470 - 5476. https://doi.org/10.1016/j.vaccine.2008.07.059
Sopp, Paul ; Coad, Michael ; Hewinson, Glyn R ; Howard, Chris John ; Cheallaigh, Cliona Ni ; Keane, Joseph ; Harris, James ; Hope, Jayne C. / Development of a simple, sensitive, rapid test which discriminates BCG-vaccinated from Mycobacterium bovis-infected cattle. In: Vaccine. 2008 ; Vol. 26, No. 43. pp. 5470 - 5476.
@article{1c337a13d8a04656aa143074ec55a159,
title = "Development of a simple, sensitive, rapid test which discriminates BCG-vaccinated from Mycobacterium bovis-infected cattle",
abstract = "Bovine tuberculosis (bTB) is increasing in incidence in the UK. Effective control strategies could involve vaccination; BCG, either alone or in prime-boost strategies, remains the most effective vaccine against bovine tuberculosis. However, BCG vaccination of cattle would require development of diagnostic tests able to accurately discriminate Mycobacterium bovis-infected from BCG-vaccinated animals. Herein, we demonstrate that the detection of secreted IFN-gamma following short term culture (4h) of whole blood with purified protein derived from M. bovis (PPD-B) allows such discrimination. This reflects, in part, the differential kinetics of IFN-gamma secretion in infected compared to vaccinated cattle. This is the first study to demonstrate that accurate, rapid distinction of BCG-vaccinated from M. bovis-infected cattle can be achieved in a short time period without the need for production of M. bovis-specific antigens, complex antigen mixtures or extensive laboratory procedures. We were also able to detect PPD-specific IFN-gamma release during short term culture of blood from a number of humans with active TB indicating that this test may have wider application and is potentially useful for the rapid diagnosis of disease in humans.",
author = "Paul Sopp and Michael Coad and Hewinson, {Glyn R} and Howard, {Chris John} and Cheallaigh, {Cliona Ni} and Joseph Keane and James Harris and Hope, {Jayne C}",
year = "2008",
doi = "10.1016/j.vaccine.2008.07.059",
language = "English",
volume = "26",
pages = "5470 -- 5476",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier",
number = "43",

}

Sopp, P, Coad, M, Hewinson, GR, Howard, CJ, Cheallaigh, CN, Keane, J, Harris, J & Hope, JC 2008, 'Development of a simple, sensitive, rapid test which discriminates BCG-vaccinated from Mycobacterium bovis-infected cattle' Vaccine, vol. 26, no. 43, pp. 5470 - 5476. https://doi.org/10.1016/j.vaccine.2008.07.059

Development of a simple, sensitive, rapid test which discriminates BCG-vaccinated from Mycobacterium bovis-infected cattle. / Sopp, Paul; Coad, Michael; Hewinson, Glyn R; Howard, Chris John; Cheallaigh, Cliona Ni; Keane, Joseph; Harris, James; Hope, Jayne C.

In: Vaccine, Vol. 26, No. 43, 2008, p. 5470 - 5476.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Development of a simple, sensitive, rapid test which discriminates BCG-vaccinated from Mycobacterium bovis-infected cattle

AU - Sopp, Paul

AU - Coad, Michael

AU - Hewinson, Glyn R

AU - Howard, Chris John

AU - Cheallaigh, Cliona Ni

AU - Keane, Joseph

AU - Harris, James

AU - Hope, Jayne C

PY - 2008

Y1 - 2008

N2 - Bovine tuberculosis (bTB) is increasing in incidence in the UK. Effective control strategies could involve vaccination; BCG, either alone or in prime-boost strategies, remains the most effective vaccine against bovine tuberculosis. However, BCG vaccination of cattle would require development of diagnostic tests able to accurately discriminate Mycobacterium bovis-infected from BCG-vaccinated animals. Herein, we demonstrate that the detection of secreted IFN-gamma following short term culture (4h) of whole blood with purified protein derived from M. bovis (PPD-B) allows such discrimination. This reflects, in part, the differential kinetics of IFN-gamma secretion in infected compared to vaccinated cattle. This is the first study to demonstrate that accurate, rapid distinction of BCG-vaccinated from M. bovis-infected cattle can be achieved in a short time period without the need for production of M. bovis-specific antigens, complex antigen mixtures or extensive laboratory procedures. We were also able to detect PPD-specific IFN-gamma release during short term culture of blood from a number of humans with active TB indicating that this test may have wider application and is potentially useful for the rapid diagnosis of disease in humans.

AB - Bovine tuberculosis (bTB) is increasing in incidence in the UK. Effective control strategies could involve vaccination; BCG, either alone or in prime-boost strategies, remains the most effective vaccine against bovine tuberculosis. However, BCG vaccination of cattle would require development of diagnostic tests able to accurately discriminate Mycobacterium bovis-infected from BCG-vaccinated animals. Herein, we demonstrate that the detection of secreted IFN-gamma following short term culture (4h) of whole blood with purified protein derived from M. bovis (PPD-B) allows such discrimination. This reflects, in part, the differential kinetics of IFN-gamma secretion in infected compared to vaccinated cattle. This is the first study to demonstrate that accurate, rapid distinction of BCG-vaccinated from M. bovis-infected cattle can be achieved in a short time period without the need for production of M. bovis-specific antigens, complex antigen mixtures or extensive laboratory procedures. We were also able to detect PPD-specific IFN-gamma release during short term culture of blood from a number of humans with active TB indicating that this test may have wider application and is potentially useful for the rapid diagnosis of disease in humans.

U2 - 10.1016/j.vaccine.2008.07.059

DO - 10.1016/j.vaccine.2008.07.059

M3 - Article

VL - 26

SP - 5470

EP - 5476

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 43

ER -