Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, induces strong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blot test was developed to serologically examine chicken serum samples for the presence of IBV antibody. Initially, 72serum samples were tested for the presence of IBV antibody using a commercial enzyme linked immunosorbent assay(ELISA) kit. Forty six IBV positive serum samples (group A) produced strong signals in the dot-blot assay. Seven negative serum samples (group B) produced weak but specific signals using the dot-blot assay in conjunction withWestern blot analysis. The remaining 19 samples (group C) from IBV negative specific pathogen free (SPF) chickens did not produce visible signals using the dot-blot assay. In conclusion, the above results suggest that the dot-blot assay is a reliable, sensitive, and specific test for serological detection of IBV positive chickens.
|Number of pages||4|
|Journal||Iranian Journal of Veterinary Research|
|Publication status||Published - 2013|
- Dot-blot hybridization
- Infectious bronchitis virus
- Nucleocapsid protein