Development of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus

H. Akrami, A. Hedayati, M. Farshian, G. Haqshenas

Research output: Contribution to journalArticleOtherpeer-review

2 Citations (Scopus)

Abstract

Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, induces strong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blot test was developed to serologically examine chicken serum samples for the presence of IBV antibody. Initially, 72serum samples were tested for the presence of IBV antibody using a commercial enzyme linked immunosorbent assay(ELISA) kit. Forty six IBV positive serum samples (group A) produced strong signals in the dot-blot assay. Seven negative serum samples (group B) produced weak but specific signals using the dot-blot assay in conjunction withWestern blot analysis. The remaining 19 samples (group C) from IBV negative specific pathogen free (SPF) chickens did not produce visible signals using the dot-blot assay. In conclusion, the above results suggest that the dot-blot assay is a reliable, sensitive, and specific test for serological detection of IBV positive chickens.

Original languageEnglish
Pages (from-to)350-353
Number of pages4
JournalIranian Journal of Veterinary Research
Volume14
Issue number4
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Dot-blot hybridization
  • Infectious bronchitis virus
  • Nucleocapsid protein

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