Abstract
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, induces strong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blot test was developed to serologically examine chicken serum samples for the presence of IBV antibody. Initially, 72serum samples were tested for the presence of IBV antibody using a commercial enzyme linked immunosorbent assay(ELISA) kit. Forty six IBV positive serum samples (group A) produced strong signals in the dot-blot assay. Seven negative serum samples (group B) produced weak but specific signals using the dot-blot assay in conjunction withWestern blot analysis. The remaining 19 samples (group C) from IBV negative specific pathogen free (SPF) chickens did not produce visible signals using the dot-blot assay. In conclusion, the above results suggest that the dot-blot assay is a reliable, sensitive, and specific test for serological detection of IBV positive chickens.
| Original language | English |
|---|---|
| Pages (from-to) | 350-353 |
| Number of pages | 4 |
| Journal | Iranian Journal of Veterinary Research |
| Volume | 14 |
| Issue number | 4 |
| Publication status | Published - 2013 |
| Externally published | Yes |
Keywords
- Dot-blot hybridization
- Infectious bronchitis virus
- Nucleocapsid protein