Development of a Novel Fluorophore for Real-Time Biomonitoring System

Hyun-Ok Song, Binna Lee, Ram Prasad Bhusal, Byounghun Park, Kyoungsik Yu, Chom Kyu Chong, Pyo Yun Cho, Sung Yeon Kim, Hak Sung Kim, Hyun Park

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Rapid in-field diagnosis is very important to prevent the outbreak of various infectious and contagious diseases. Highly sensitive and quantitative detection of diseases can be performed using fluorescent immunochemical assay with specific antigen-antibody binding and a good quality fluorophore. This can lead to the development of a small, portable, quantitative biosensor to transmit diagnostic results to a control center in order to systematically prevent disease outbreaks. In this study, we developed a novel fluorophore, coumarin-derived dendrimer, with high emission intensity, strong signal brightness, and high photostability. It is easily coupled with biomolecules and emits strong and stable fluorescence at 590 nm with excitation at 455 nm. Application to fluorescent immunochromatographic test (FICT) showed that the novel coumarin-derived dendrimer bioconjugate could detect antigens at amount as low as 0.1 ng. The clinical results and the spectral characteristics of the novel coumarin-derived dendrimer open, for the first time, the possibility of developing a cost/energy efficient LED-based portable quantitative biosensor for point-of-care (POC) disease diagnosis, which can permit real time monitoring (U-healthcare system) by a disease control center.

Original languageEnglish
Article numbere48459
Number of pages10
JournalPLoS ONE
Volume7
Issue number11
DOIs
Publication statusPublished - 2 Nov 2012
Externally publishedYes

Keywords

  • plasmodium
  • malarial parasites
  • diagnostic medicine
  • malaria
  • fluorescence
  • fluorescent dyes
  • coumarins
  • infectious disease control

Cite this

Song, H-O., Lee, B., Bhusal, R. P., Park, B., Yu, K., Chong, C. K., ... Park, H. (2012). Development of a Novel Fluorophore for Real-Time Biomonitoring System. PLoS ONE, 7(11), [e48459]. https://doi.org/10.1371/journal.pone.0048459
Song, Hyun-Ok ; Lee, Binna ; Bhusal, Ram Prasad ; Park, Byounghun ; Yu, Kyoungsik ; Chong, Chom Kyu ; Cho, Pyo Yun ; Kim, Sung Yeon ; Kim, Hak Sung ; Park, Hyun. / Development of a Novel Fluorophore for Real-Time Biomonitoring System. In: PLoS ONE. 2012 ; Vol. 7, No. 11.
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Song, H-O, Lee, B, Bhusal, RP, Park, B, Yu, K, Chong, CK, Cho, PY, Kim, SY, Kim, HS & Park, H 2012, 'Development of a Novel Fluorophore for Real-Time Biomonitoring System', PLoS ONE, vol. 7, no. 11, e48459. https://doi.org/10.1371/journal.pone.0048459

Development of a Novel Fluorophore for Real-Time Biomonitoring System. / Song, Hyun-Ok; Lee, Binna; Bhusal, Ram Prasad; Park, Byounghun; Yu, Kyoungsik; Chong, Chom Kyu; Cho, Pyo Yun; Kim, Sung Yeon; Kim, Hak Sung; Park, Hyun.

In: PLoS ONE, Vol. 7, No. 11, e48459, 02.11.2012.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Song, Hyun-Ok

AU - Lee, Binna

AU - Bhusal, Ram Prasad

AU - Park, Byounghun

AU - Yu, Kyoungsik

AU - Chong, Chom Kyu

AU - Cho, Pyo Yun

AU - Kim, Sung Yeon

AU - Kim, Hak Sung

AU - Park, Hyun

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KW - malarial parasites

KW - diagnostic medicine

KW - malaria

KW - fluorescence

KW - fluorescent dyes

KW - coumarins

KW - infectious disease control

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