Objective: To develop a method for enriching methylated DNA in clinical samples using mesocellular silica foams (MCFs) immobilized with methyl-CpG binding domain (MBD). Methods: MCFs with ultra-large pore size were synthesized, functionalized and immobilized with GST-MBD. Results: The large cage-like pore structures of MCF materials was retained after functionalization and immobilization, with pore diameter of 55 nm, window size of 30 nm, and a high pore volume of 1.0 cm3/g. The loading amount of MBD was as high as 53 wt%. Immobilized MBD showed high binding activity and stability. In a binding buffer with salt concentrations ranging 500-550 mmol/L, the MCF-MBD can selectively enrich methylated DNA from the mixed DNA solution. Conclusion: The MCF-MBD method may offer a better choice for high-throughout DNA methylation screening, and has laid a foundation for clinical application, prenatal diagnosis and research on DNA methylation-related genetic diseases.
|Number of pages||5|
|Journal||Chinese Journal of Medical Genetics|
|Publication status||Published - 10 Jun 2012|
- Functionalized mesocellular silica foams
- Methyl-CpG binding domain
- Methylated DNA