Development of a method for methylated DNA enrichment with functionalized mesocellular silica foams immobilized with methyl CpG binding domain

Ya Ting Chen, Lu Han, Dong Yuan Zhao, Bo Tu, Duan Ma

Research output: Contribution to journalArticleResearchpeer-review


Objective: To develop a method for enriching methylated DNA in clinical samples using mesocellular silica foams (MCFs) immobilized with methyl-CpG binding domain (MBD). Methods: MCFs with ultra-large pore size were synthesized, functionalized and immobilized with GST-MBD. Results: The large cage-like pore structures of MCF materials was retained after functionalization and immobilization, with pore diameter of 55 nm, window size of 30 nm, and a high pore volume of 1.0 cm3/g. The loading amount of MBD was as high as 53 wt%. Immobilized MBD showed high binding activity and stability. In a binding buffer with salt concentrations ranging 500-550 mmol/L, the MCF-MBD can selectively enrich methylated DNA from the mixed DNA solution. Conclusion: The MCF-MBD method may offer a better choice for high-throughout DNA methylation screening, and has laid a foundation for clinical application, prenatal diagnosis and research on DNA methylation-related genetic diseases.

Original languageEnglish
Pages (from-to)284-288
Number of pages5
JournalChinese Journal of Medical Genetics
Issue number3
Publication statusPublished - 10 Jun 2012
Externally publishedYes


  • Enrichment
  • Functionalized mesocellular silica foams
  • Methyl-CpG binding domain
  • Methylated DNA

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