Determination of vitamin B1 in pharmaceutical preparations by flow-injection analysis with biamperometric detection

Li Jun Li, Hao Cheng, Chuan Zhao, Xiao Ge Hu

Research output: Contribution to journalArticleResearchpeer-review


A new electrochemical method for the on-line determination of vitamin B-1 is presented. Based on dead-stop end-point biamperometry by using two platinum foil electrodes with an applied potential difference of 150 mV, vitamin B-1 can be oxidized by hexacyanoferrate (III) in a sodium, hydroxide medium via a reversible indicating couple Fe(CN64-/Fe(CN)(6)(3-). The cell current is linear with the concentration of vitamin B-1 in the concentration range of 4.0 X 10(-6) - 1. 0 X 10(-1) mol/L with a detection limit of 8.0 X 10(-7) mol/L(0.27 mu g/mL). Most familiar excipients, ions and vitamins do not interfere with the determination of vitamin B-1. The method displays the advantages of simplicity, high efficiency( 180 samples/h), and high selectivity, and is suitable for the determination of vitamin B-1 in pharmaceutical preparations.
Original languageEnglish
Pages (from-to)650 - 653
Number of pages4
JournalChemical Research in Chinese Universities
Issue number6
Publication statusPublished - 2005
Externally publishedYes

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