A new electrochemical method for the on-line determination of vitamin B-1 is presented. Based on dead-stop end-point biamperometry by using two platinum foil electrodes with an applied potential difference of 150 mV, vitamin B-1 can be oxidized by hexacyanoferrate (III) in a sodium, hydroxide medium via a reversible indicating couple Fe(CN64-/Fe(CN)(6)(3-). The cell current is linear with the concentration of vitamin B-1 in the concentration range of 4.0 X 10(-6) - 1. 0 X 10(-1) mol/L with a detection limit of 8.0 X 10(-7) mol/L(0.27 mu g/mL). Most familiar excipients, ions and vitamins do not interfere with the determination of vitamin B-1. The method displays the advantages of simplicity, high efficiency( 180 samples/h), and high selectivity, and is suitable for the determination of vitamin B-1 in pharmaceutical preparations.
|Pages (from-to)||650 - 653|
|Number of pages||4|
|Journal||Chemical Research in Chinese Universities|
|Publication status||Published - 2005|