TY - JOUR
T1 - Determination of the sub-lethal nephrotoxic dose of Russell's viper (Daboia russelii) venom in Wistar rats
AU - Wijewickrama, Eranga S.
AU - Kurukulasooriya, Ishani
AU - Gunatilake, Mangala
AU - Priyani, Amarathunga AH
AU - Gnanathasan, Ariaranee
AU - Gawarammana, Indika
AU - Isbister, Geoffrey K.
PY - 2018/9/15
Y1 - 2018/9/15
N2 - Wistar rats were administered increasing doses of Russell's viper venom (RVV; 0.025–0.4 mg/kg) intraperitoneally to investigate acute kidney injury (AKI) by measuring creatinine (1.5-fold increase in serum creatinine above baseline) and examining kidney histology. Approximately 50% of rats receiving 0.25–0.4 mg/kg venom died within 72 h. An increase in serum creatinine only occurred at a venom dose of 0.4 mg/kg, except in two rats. Acute tubular necrosis, glomerular necrosis, cortical necrosis and interstitial inflammation were observed at venom doses of ≥0.25 mg/kg in 12/36 rats. However, of those 12 rats only four survived to 48 h compared to the 24 rats not developing nephrotoxicity, in which 18 were alive at 48 h. There was poor correlation between histological nephrotoxicity and AKI based on creatinine measurement. The early death in rats with AKI makes this a poor model for studying RVV-induced AKI.
AB - Wistar rats were administered increasing doses of Russell's viper venom (RVV; 0.025–0.4 mg/kg) intraperitoneally to investigate acute kidney injury (AKI) by measuring creatinine (1.5-fold increase in serum creatinine above baseline) and examining kidney histology. Approximately 50% of rats receiving 0.25–0.4 mg/kg venom died within 72 h. An increase in serum creatinine only occurred at a venom dose of 0.4 mg/kg, except in two rats. Acute tubular necrosis, glomerular necrosis, cortical necrosis and interstitial inflammation were observed at venom doses of ≥0.25 mg/kg in 12/36 rats. However, of those 12 rats only four survived to 48 h compared to the 24 rats not developing nephrotoxicity, in which 18 were alive at 48 h. There was poor correlation between histological nephrotoxicity and AKI based on creatinine measurement. The early death in rats with AKI makes this a poor model for studying RVV-induced AKI.
KW - Acute kidney injury
KW - Nephrotoxic venom dose
KW - Russell's viper
KW - Wistar rat
UR - https://www.scopus.com/pages/publications/85053072863
U2 - 10.1016/j.toxicon.2018.07.023
DO - 10.1016/j.toxicon.2018.07.023
M3 - Article
AN - SCOPUS:85053072863
SN - 0041-0101
VL - 152
SP - 43
EP - 45
JO - Toxicon
JF - Toxicon
ER -