Determination of lamotrigine in human plasma by high-performance liquid chromatography

Pela Angelis-Stoforidis, Denis J. Morgan, Terence J. O'Brien, Frank J E Vajda

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The method involves precipitation of plasma proteins with acetonitrile and analysis of the supernatant by high-performance liquid chromatography using a 5 μm Zorbax C8 column. Quantitation was performed by measurement of the UV absorbance at a wavelength of 306 nm. The method was linear in the range of 1-20 μg/ml, with a mean coefficient of determination (r2=0.998). The limit of detection was 0.6 μg/ml and the lower limit of quantitation was 1 μg/ml using 200 μl of plasma. Within- and between-day accuracy and precision were below 6% at all analysed concentrations except at the limit of quantitation. No interfering peaks were found by commonly monitored antiepileptic drugs. Recovery was found to be ≥99%. Satisfactory performance was obtained in the evaluation of epileptic patient samples, whose results of plasma concentration measurements are briefly discussed. We conclude that this is a reliable method for the routine monitoring of lamotrigine concentration in plasma in the clinical setting. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number1-2
Publication statusPublished - 30 Apr 1999


  • Lamotrigine

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