The method involves precipitation of plasma proteins with acetonitrile and analysis of the supernatant by high-performance liquid chromatography using a 5 μm Zorbax C8 column. Quantitation was performed by measurement of the UV absorbance at a wavelength of 306 nm. The method was linear in the range of 1-20 μg/ml, with a mean coefficient of determination (r2=0.998). The limit of detection was 0.6 μg/ml and the lower limit of quantitation was 1 μg/ml using 200 μl of plasma. Within- and between-day accuracy and precision were below 6% at all analysed concentrations except at the limit of quantitation. No interfering peaks were found by commonly monitored antiepileptic drugs. Recovery was found to be ≥99%. Satisfactory performance was obtained in the evaluation of epileptic patient samples, whose results of plasma concentration measurements are briefly discussed. We conclude that this is a reliable method for the routine monitoring of lamotrigine concentration in plasma in the clinical setting. Copyright (C) 1999 Elsevier Science B.V.
|Number of pages||6|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|Publication status||Published - 30 Apr 1999|