Abstract
A simple high-performance liquid chromatography (HPLC) method to determine the content of betulinic acid (BA) in rat plasma collected at different times (0-8 h) after oral administration of Orthosiphon stamineus leaf extract was developed. The features of the assay include protein precipitation using acetonitrile and isocratic elution using reverse phase C-18 column with ultraviolet (UV) detection. The recovery of BA from plasma varied from 98.4 to 102.5%. The R.S.D of intra- and inter-day precision from rat plasma ranged from 4.2 to 9.8%. The maximum concentration of BA in the plasma was 1.2 ± 0.3 μg/ml at 1 h after oral administration of the extract.
| Original language | English |
|---|---|
| Pages (from-to) | 788-790 |
| Number of pages | 3 |
| Journal | Pharmazie |
| Volume | 63 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 2008 |
| Externally published | Yes |