Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting

Nicole Kerlero De Rosbo, Patrick R. Carnegie, Claude C.A. Bernard, D. Scott Linthicum

Research output: Contribution to journalArticleResearchpeer-review

40 Citations (Scopus)

Abstract

An electroimmunoblot technique was used to detect various forms of myelin basic protein (MBP) in brain homogenates of 14 vertebrate species. Three antibodies were used to probe the immunoblots: a monoclonal anti-human MPB reacting with an antigenic determinant located at amino acid residues 131 to 136; a polyclonal anti-human MBP and a polyclonal anti-chicken MBP. Because no processing of the tissue is required prior to electrophoresis, in vitro artifacts are minimized. The 18.5 K form of MBP was present in all species except the shark. A 21.5 K MBP was observed in ovine, bovine, pig, rabbit, mouse, rat, monkey, but not in human, guinea pig, shark, toad and marsupial brains. A variant with a molecular weight between 17 K and 18 K was found in mouse, rat, bovine, human, monkey, pig, and chicken brains, and was the sole component in the shark brain. Marsupial brains had five or six forms of MBP between 14.5 K and 18.5 K.

Original languageEnglish
Pages (from-to)1359-1369
Number of pages11
JournalNeurochemical Research
Volume9
Issue number10
DOIs
Publication statusPublished - Oct 1984
Externally publishedYes

Cite this