Detection of integrin-linked kinase in the serum of patients with malignant pleural mesothelioma

Stefan Watzka, Florian Posch, Harvey Pass, Margaret Huflejt, David Bernhard, Gregory Hannigan, Michael Muller

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Abstract

Integrin-linked kinase, which is relevant to neoplastic transformation, is highly expressed in malignant pleural mesothelioma. Recently, detection of integrin-linked kinase in serum of patients with ovarian cancer has been reported. This study asks whether integrin-linked kinase can also be detected in serum of patients with malignant pleural mesothelioma and whether serum level has diagnostic or prognostic relevance for that disease. METHODS: A sandwich enzyme-linked immunosorbent assay was designed to detect integrin-linked kinase and applied to serum samples from 46 patients with malignant pleural mesothelioma, 98 patients with other malignant chest disease, and 23 patients with benign chest disease. Integrin-linked kinase serum concentration and clinical data were correlated statistically. RESULTS: Median serum integrin-linked kinase concentration was significantly higher in malignant pleural mesothelioma (8.89 ng/mL) than in other malignant chest disease (0.66 ng/mL) or benign chest disease (0.78 ng/mL, P <.001). There was no relevant correlation of serum integrin-linked kinase with cell lysis parameters (R(2) <0.1). Serum integrin-linked kinase concentration greater than 2.48 ng/mL had diagnostic sensitivity of 80 , specificity of 95 , positive predictive value of 85.7 , negative predictive value of 92.7 , and overall accuracy of 91 for distinction between malignant pleural mesothelioma and other diseases. Serum integrin-linked kinase concentration in malignant pleural mesothelioma was independent of histologic subtype or asbestos exposure. There was no statistically significant impact of serum integrin-linked kinase concentration on prognosis. CONCLUSIONS: Integrin-linked kinase can be detected in serum of patients with malignant pleural mesothelioma and may be a diagnostic marker for the disease.
Original languageEnglish
Pages (from-to)384 - 389
Number of pages6
JournalJournal of Thoracic and Cardiovascular Surgery
Volume142
Issue number2
DOIs
Publication statusPublished - 2011

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