Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction

B. Chaharaein, A. R. Omar, I. Aini, K. Yusoff, S. S. Hassan

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32 Citations (Scopus)

Abstract

Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR-enzyme-linked immunosorbent assay (ELISA) and conventional RT-PCR. While the sensitivity of the multiplex RT-PCR is as sensitive as the conventional RT-PCR, it is 10 times less sensitive than RT-PCR-ELISA. The multiplex RT-PCR is also as sensitive as the virus isolation method in detecting H9N2 from tracheal samples collected at day 3 and 5 post inoculation. Hence, the developed multiplex RT-PCR assay is a rapid, sensitive and specific assay for detecting of AIV subtypes.

Original languageEnglish
Pages (from-to)174-179
Number of pages6
JournalMicrobiological Research
Volume164
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • H9N2 subtype
  • Multiplex RT-PCR
  • Type A avian influenza virus infections

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