Analysis of gene expression patterns is central to the study of embryonic development. This chapter details methods for detecting gene expression in whole mouse embryos and in tissue sections. The most commonly used methods available in mouse are described and include mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters.
|Title of host publication||Methods in Molecular Biology - Vertebrate Embryogenesis|
|Editors||F J Pelegri|
|Place of Publication||USA|
|Pages||259 - 292|
|Number of pages||34|
|Publication status||Published - 2011|