Detection of Gene Expression in Mouse Embryos and Tissue Sections

Edwina McGlinn; Jennifer Mansfield

doi:10.1007/978-1-61779-210-6_10

Detection of Gene Expression in Mouse Embryos and Tissue Sections

Edwina McGlinn, Jennifer Mansfield

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

15 Citations (Scopus)

Abstract

Analysis of gene expression patterns is central to the study of embryonic development. This chapter details methods for detecting gene expression in whole mouse embryos and in tissue sections. The most commonly used methods available in mouse are described and include mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters.

Original language	English
Title of host publication	Methods in Molecular Biology - Vertebrate Embryogenesis
Editors	F J Pelegri
Place of Publication	USA
Publisher	Humana Press
Pages	259 - 292
Number of pages	34
Edition	1st
ISBN (Print)	9781617792090
DOIs	https://doi.org/10.1007/978-1-61779-210-6_10
Publication status	Published - 2011
Externally published	Yes

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10.1007/978-1-61779-210-6_10

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Detection of Gene Expression in Mouse Embryos and Tissue Sections

Abstract

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