Detection of Gene Expression in Mouse Embryos and Tissue Sections

Edwina McGlinn, Jennifer Mansfield

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

10 Citations (Scopus)

Abstract

Analysis of gene expression patterns is central to the study of embryonic development. This chapter details methods for detecting gene expression in whole mouse embryos and in tissue sections. The most commonly used methods available in mouse are described and include mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters.
Original languageEnglish
Title of host publicationMethods in Molecular Biology - Vertebrate Embryogenesis
EditorsF J Pelegri
Place of PublicationUSA
PublisherHumana Press
Pages259 - 292
Number of pages34
Edition1st
ISBN (Print)9781617792090
DOIs
Publication statusPublished - 2011
Externally publishedYes

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