Detection of gene and protein expression in mouse embryos and tissue sections

Edwina McGlinn, Miriam A. Holzman, Jennifer H. Mansfield

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.

Original languageEnglish
Title of host publicationVertebrate Embryogenesis
EditorsFrancisco J. Pelegri
Place of PublicationNew York, NY
PublisherHumana Press
Chapter12
Pages183-218
Number of pages36
ISBN (Electronic)9781493990092
ISBN (Print)9781493990085
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume1920
ISSN (Print)1064-3745

Keywords

  • Alkaline phosphatase
  • Green fluorescent protein
  • Immunohistochemistry
  • In situ hybridization
  • Mouse embryogenesis
  • ß-Galactosidase

Cite this

McGlinn, E., Holzman, M. A., & Mansfield, J. H. (2019). Detection of gene and protein expression in mouse embryos and tissue sections. In F. J. Pelegri (Ed.), Vertebrate Embryogenesis (pp. 183-218). (Methods in Molecular Biology; Vol. 1920). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-9009-2_12
McGlinn, Edwina ; Holzman, Miriam A. ; Mansfield, Jennifer H. / Detection of gene and protein expression in mouse embryos and tissue sections. Vertebrate Embryogenesis. editor / Francisco J. Pelegri. New York, NY : Humana Press, 2019. pp. 183-218 (Methods in Molecular Biology).
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McGlinn, E, Holzman, MA & Mansfield, JH 2019, Detection of gene and protein expression in mouse embryos and tissue sections. in FJ Pelegri (ed.), Vertebrate Embryogenesis. Methods in Molecular Biology, vol. 1920, Humana Press, New York, NY, pp. 183-218. https://doi.org/10.1007/978-1-4939-9009-2_12

Detection of gene and protein expression in mouse embryos and tissue sections. / McGlinn, Edwina; Holzman, Miriam A.; Mansfield, Jennifer H.

Vertebrate Embryogenesis. ed. / Francisco J. Pelegri. New York, NY : Humana Press, 2019. p. 183-218 (Methods in Molecular Biology; Vol. 1920).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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McGlinn E, Holzman MA, Mansfield JH. Detection of gene and protein expression in mouse embryos and tissue sections. In Pelegri FJ, editor, Vertebrate Embryogenesis. New York, NY: Humana Press. 2019. p. 183-218. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9009-2_12