Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigenin-labelled probe

A. M.Y. Permeen; C. K. Sam; R. Pathmanathan; U. Prasad; H. Wolf

doi:10.1016/0166-0934(90)90094-V

Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigenin-labelled probe

A. M.Y. Permeen, C. K. Sam, R. Pathmanathan, U. Prasad, H. Wolf

Research output: Contribution to journal › Article › Research › peer-review

13 Citations (Scopus)

Abstract

The presence of Epstein Barr vims (EBV) DNA in biopsies from the post-nasal space (PNS) of patients suspected of nasopharyngeal carcinoma (NPC) was detected by in situ cytohybridisation with an EBV DNA probe labelled with the novel labelling compound digoxigenin. The digoxigenin probe was hybridised to cryostat sections of NPC biopsies and subsequently detected by an enzyme immunoassay procedure. It was found that in situ cytohybridisation using the digoxigenin probe was much more rapid and sensitive (96 h compared to five weeks) than the current method of using ³H-labelled probe. Using the digoxigenin EBV probe, it was found that in all the eighteen NPC biopsies tested, EBV DNA was detected in malignant epithelial cells and infiltrating lymphocytes. EBV DNA was also detected in some normal epithelial cells in these NPC biopsies. EBV DNA was not detected in epithelial cells of non-malignant biopsies.

Original language	English
Pages (from-to)	261-267
Number of pages	7
Journal	Journal of Virological Methods
Volume	27
Issue number	3
DOIs	https://doi.org/10.1016/0166-0934(90)90094-V
Publication status	Published - Mar 1990
Externally published	Yes

Keywords

Digoxigenin
Epstein-Barr virus
Nasopharyngeal carcinoma

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10.1016/0166-0934(90)90094-V

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@article{f2940d0285844fd0989a68649415c061,

title = "Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigenin-labelled probe",

abstract = "The presence of Epstein Barr vims (EBV) DNA in biopsies from the post-nasal space (PNS) of patients suspected of nasopharyngeal carcinoma (NPC) was detected by in situ cytohybridisation with an EBV DNA probe labelled with the novel labelling compound digoxigenin. The digoxigenin probe was hybridised to cryostat sections of NPC biopsies and subsequently detected by an enzyme immunoassay procedure. It was found that in situ cytohybridisation using the digoxigenin probe was much more rapid and sensitive (96 h compared to five weeks) than the current method of using 3H-labelled probe. Using the digoxigenin EBV probe, it was found that in all the eighteen NPC biopsies tested, EBV DNA was detected in malignant epithelial cells and infiltrating lymphocytes. EBV DNA was also detected in some normal epithelial cells in these NPC biopsies. EBV DNA was not detected in epithelial cells of non-malignant biopsies.",

keywords = "Digoxigenin, Epstein-Barr virus, Nasopharyngeal carcinoma",

author = "Permeen, {A. M.Y.} and Sam, {C. K.} and R. Pathmanathan and U. Prasad and H. Wolf",

year = "1990",

month = mar,

doi = "10.1016/0166-0934(90)90094-V",

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pages = "261--267",

journal = "Journal of Virological Methods",

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T1 - Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigenin-labelled probe

AU - Permeen, A. M.Y.

AU - Sam, C. K.

AU - Pathmanathan, R.

AU - Prasad, U.

AU - Wolf, H.

PY - 1990/3

Y1 - 1990/3

N2 - The presence of Epstein Barr vims (EBV) DNA in biopsies from the post-nasal space (PNS) of patients suspected of nasopharyngeal carcinoma (NPC) was detected by in situ cytohybridisation with an EBV DNA probe labelled with the novel labelling compound digoxigenin. The digoxigenin probe was hybridised to cryostat sections of NPC biopsies and subsequently detected by an enzyme immunoassay procedure. It was found that in situ cytohybridisation using the digoxigenin probe was much more rapid and sensitive (96 h compared to five weeks) than the current method of using 3H-labelled probe. Using the digoxigenin EBV probe, it was found that in all the eighteen NPC biopsies tested, EBV DNA was detected in malignant epithelial cells and infiltrating lymphocytes. EBV DNA was also detected in some normal epithelial cells in these NPC biopsies. EBV DNA was not detected in epithelial cells of non-malignant biopsies.

AB - The presence of Epstein Barr vims (EBV) DNA in biopsies from the post-nasal space (PNS) of patients suspected of nasopharyngeal carcinoma (NPC) was detected by in situ cytohybridisation with an EBV DNA probe labelled with the novel labelling compound digoxigenin. The digoxigenin probe was hybridised to cryostat sections of NPC biopsies and subsequently detected by an enzyme immunoassay procedure. It was found that in situ cytohybridisation using the digoxigenin probe was much more rapid and sensitive (96 h compared to five weeks) than the current method of using 3H-labelled probe. Using the digoxigenin EBV probe, it was found that in all the eighteen NPC biopsies tested, EBV DNA was detected in malignant epithelial cells and infiltrating lymphocytes. EBV DNA was also detected in some normal epithelial cells in these NPC biopsies. EBV DNA was not detected in epithelial cells of non-malignant biopsies.

KW - Digoxigenin

KW - Epstein-Barr virus

KW - Nasopharyngeal carcinoma

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ER -

Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigenin-labelled probe

Abstract

Keywords

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