Detection of double stranded RNA in Semliki Forest virus infected cells by an indirect solid phase radioimmunoassay: an assay for interferon

B. R.G. Williams; J. Kalmakoff

doi:10.1159/000148885

Detection of double stranded RNA in Semliki Forest virus infected cells by an indirect solid phase radioimmunoassay: an assay for interferon

B. R.G. Williams, J. Kalmakoff

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

An indirect solid phase radioimmunoassay using rabbit antibodies to the synthetic double-stranded RNA poly rIrC and ¹²⁵I-labelled sheep anti-rabbit IgG could detect 1 ng of poly rIrC. This assay could also detect the presence of double-stranded RNA in Semliki Forest virus-infected chick fibroblasts and can be used as an assay for interferon activity.

Original language	English
Pages (from-to)	110-116
Number of pages	7
Journal	Intervirology
Volume	8
Issue number	2
DOIs	https://doi.org/10.1159/000148885
Publication status	Published - 1 Jan 1977
Externally published	Yes

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10.1159/000148885

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title = "Detection of double stranded RNA in Semliki Forest virus infected cells by an indirect solid phase radioimmunoassay: an assay for interferon",

abstract = "An indirect solid phase radioimmunoassay using rabbit antibodies to the synthetic double-stranded RNA poly rIrC and 125I-labelled sheep anti-rabbit IgG could detect 1 ng of poly rIrC. This assay could also detect the presence of double-stranded RNA in Semliki Forest virus-infected chick fibroblasts and can be used as an assay for interferon activity.",

author = "Williams, {B. R.G.} and J. Kalmakoff",

year = "1977",

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T2 - an assay for interferon

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AU - Kalmakoff, J.

PY - 1977/1/1

Y1 - 1977/1/1

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AB - An indirect solid phase radioimmunoassay using rabbit antibodies to the synthetic double-stranded RNA poly rIrC and 125I-labelled sheep anti-rabbit IgG could detect 1 ng of poly rIrC. This assay could also detect the presence of double-stranded RNA in Semliki Forest virus-infected chick fibroblasts and can be used as an assay for interferon activity.

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U2 - 10.1159/000148885

DO - 10.1159/000148885

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SP - 110

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JF - Intervirology

SN - 0300-5526

IS - 2

ER -

Detection of double stranded RNA in Semliki Forest virus infected cells by an indirect solid phase radioimmunoassay: an assay for interferon

Abstract

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