Detection of Active Granzyme A in NK92 Cells with Fluorescent Activity-Based Probe

Sonia Kołt, Tomasz Janiszewski, Dion Kaiserman, Sylwia Modrzycka, Scott J. Snipas, Guy Salvesen, Marcin Dra¸g, Phillip I. Bird, Paulina Kasperkiewicz

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Cytotoxic T-lymphocytes (CTLs) and natural killer cells (NKs) kill compromised cells to defend against tumor and viral infections. Both effector cell types use multiple strategies to induce target cell death including Fas/CD95 activation and the release of perforin and a group of lymphocyte granule serine proteases called granzymes. Granzymes have relatively broad and overlapping substrate specificities and may hydrolyze a wide range of peptidic epitopes; it is therefore challenging to identify their natural and synthetic substrates and to distinguish their localization and functions. Here, we present a specific and potent substrate, an inhibitor, and an activity-based probe of Granzyme A (GrA) that can be used to follow functional GrA in cells.

Original languageEnglish
Pages (from-to)3359-3369
Number of pages11
JournalJournal of Medicinal Chemistry
Volume63
Issue number6
DOIs
Publication statusPublished - 26 Mar 2020

Cite this