Detection and Whole-Genome Analysis of a High-Risk Clone of Klebsiella pneumoniae ST340/CG258 Producing CTX-M-15 in a Companion Animal

Juan Valencia-Bacca, Meire M. Silva, Louise Cerdeira, Fernanda Esposito, Brenda Cardoso, Maria E. Muñoz, Tatiana Jiménez-Villegas, Adriana Cardenas-Arias, Danielle A.N. Pessoa, Nilton Lincopan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The emergence and dissemination of high-risk clones of Klebsiella pneumoniae producing extended-spectrum β-lactamases (ESBLs) in animal infections is a critical issue. We report the detection and genomic features of a multidrug-resistant (MDR) ESBL (CTX-M-15)-producing K. pneumoniae infecting a domestic cat. Whole-genome sequencing analysis identified the international ST340 (clonal group CG258), and genes and mutations conferring resistance to β-lactams, aminoglycosides, macrolides, phenicols, fosfomycin, sulfonamides, tetracycline, trimethoprim, and fluoroquinolones. In addition, the presence of genes encoding resistance to disinfectant and heavy metals hazardous to humans was also confirmed. The MDR profile exhibited by the strain contributed to treatment failure and death of the companion animal. Therefore, active surveillance of critical priority lineages of K. pneumoniae should not only focus on human infections but also on veterinary infections.

Original languageEnglish
Pages (from-to)611-615
Number of pages5
JournalMicrobial Drug Resistance
Volume26
Issue number6
DOIs
Publication statusAccepted/In press - Dec 2019
Externally publishedYes

Keywords

  • bacterial pathogens
  • Brazil
  • ESBL
  • multidrug-resistant
  • One Health
  • pets

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