Detection and serotyping of lyophilized nonculturable pneumococcal isolates

Joyanta K. Modak, Mark C. Steinhoff, Khalil Zaman, Maksuda Amir Islam, Shams E Arifeen, Samir K. Saha

Research output: Contribution to journalArticleOtherpeer-review

5 Citations (Scopus)

Abstract

One hundred fifty-two nonculturable lyophilized carriage pneumococcal isolates from a vaccine trial were subjected to PCR for serotyping, and 149 (98%) were successfully classified as vaccine or nonvaccine types, which were similar to viable isolates. The methodology will be useful for analysis of this and other studies where stored pneumococcal isolates fail to grow.
Original languageEnglish
Pages (from-to)3388-3390
Number of pages3
JournalJournal of Clinical Microbiology
Volume50
Issue number10
DOIs
Publication statusPublished - 1 Oct 2012
Externally publishedYes

Cite this