Resistance to the aminoglycosides gentamicin (Gm(r)), tobramycin (Tm(r)) and kanamycin (Km(r)) in strains of Staphylococcus aureus isolated in Australia is mediated by the transposon Tn4001. The 1.35 kb inverted repeat of this transposon exhibits many of the characteristics of an insertion sequence and has consequently been designated IS256. Tandem duplication of IS256 contiguous with Tn4001 results in an increase in the level of Gm(r)Tm(r)Km(r), thereby implying that the element possesses strong promoter sequences. Both contiguous and independent insertions of IS256 into the staphylococcal chromosome have been observed, the latter suggesting that the element may play a role in molecular rearrangements of the genome.
|Number of pages||8|
|Journal||Journal of General Microbiology|
|Publication status||Published - 1 Dec 1987|