Detecting DNasel-hypersensitivity sites with MLPA

Thomas Ohnesorg; Stephanie Eggers; Stefan White

doi:10.1007/978-1-61779-292-2_12

Detecting DNasel-hypersensitivity sites with MLPA

Thomas Ohnesorg, Stephanie Eggers, Stefan White

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

1 Citation (Scopus)

Abstract

DNaseI-hypersensitive sites within chromatin are indicative of genomic loci with regulatory function. Several techniques have been described for analyzing these regions, but are either laborious, offer low-throughput possibilities, or are expensive. We have developed a new approach based on a modified version of multiplex ligation-dependent probe amplification (MLPA). Using this method, it is possible to analyse up to 50 defined genomic regions for DNaseI-hypersensitivity in a single PCR-based reaction. This chapter outlines the approach and discusses the critical features of each step of the procedure.

Original language	English
Title of host publication	Gene Regulatory Networks : Methods and Protocols
Editors	Bart Deplancke, Nele Gheldof
Place of Publication	New York, USA
Publisher	Humana Press
Pages	201 - 210
Number of pages	10
Edition	Vol. 786
ISBN (Print)	9781617792915
DOIs	https://doi.org/10.1007/978-1-61779-292-2_12
Publication status	Published - 2012

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10.1007/978-1-61779-292-2_12

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Cite this

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abstract = "DNaseI-hypersensitive sites within chromatin are indicative of genomic loci with regulatory function. Several techniques have been described for analyzing these regions, but are either laborious, offer low-throughput possibilities, or are expensive. We have developed a new approach based on a modified version of multiplex ligation-dependent probe amplification (MLPA). Using this method, it is possible to analyse up to 50 defined genomic regions for DNaseI-hypersensitivity in a single PCR-based reaction. This chapter outlines the approach and discusses the critical features of each step of the procedure.",

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AU - Eggers, Stephanie

AU - White, Stefan

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AB - DNaseI-hypersensitive sites within chromatin are indicative of genomic loci with regulatory function. Several techniques have been described for analyzing these regions, but are either laborious, offer low-throughput possibilities, or are expensive. We have developed a new approach based on a modified version of multiplex ligation-dependent probe amplification (MLPA). Using this method, it is possible to analyse up to 50 defined genomic regions for DNaseI-hypersensitivity in a single PCR-based reaction. This chapter outlines the approach and discusses the critical features of each step of the procedure.

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