Abstract
DNaseI-hypersensitive sites within chromatin are indicative of genomic loci with regulatory function. Several techniques have been described for analyzing these regions, but are either laborious, offer low-throughput possibilities, or are expensive. We have developed a new approach based on a modified version of multiplex ligation-dependent probe amplification (MLPA). Using this method, it is possible to analyse up to 50 defined genomic regions for DNaseI-hypersensitivity in a single PCR-based reaction. This chapter outlines the approach and discusses the critical features of each step of the procedure.
Original language | English |
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Title of host publication | Gene Regulatory Networks : Methods and Protocols |
Editors | Bart Deplancke, Nele Gheldof |
Place of Publication | New York, USA |
Publisher | Humana Press |
Pages | 201 - 210 |
Number of pages | 10 |
Edition | Vol. 786 |
ISBN (Print) | 9781617792915 |
DOIs | |
Publication status | Published - 2012 |