Detecting DNasel-hypersensitivity sites with MLPA

Thomas Ohnesorg, Stephanie Eggers, Stefan White

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

    1 Citation (Scopus)

    Abstract

    DNaseI-hypersensitive sites within chromatin are indicative of genomic loci with regulatory function. Several techniques have been described for analyzing these regions, but are either laborious, offer low-throughput possibilities, or are expensive. We have developed a new approach based on a modified version of multiplex ligation-dependent probe amplification (MLPA). Using this method, it is possible to analyse up to 50 defined genomic regions for DNaseI-hypersensitivity in a single PCR-based reaction. This chapter outlines the approach and discusses the critical features of each step of the procedure.
    Original languageEnglish
    Title of host publicationGene Regulatory Networks : Methods and Protocols
    EditorsBart Deplancke, Nele Gheldof
    Place of PublicationNew York, USA
    PublisherHumana Press
    Pages201 - 210
    Number of pages10
    EditionVol. 786
    ISBN (Print)9781617792915
    DOIs
    Publication statusPublished - 2012

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