Designer Neural Networks with Embedded Semiconductor Microtube Arrays

Aune Koitmäe; Manuel Müller; Cornelius S. Bausch; Jann Harberts; Wolfgang Hansen; Gabriele Loers; Robert H. Blick

doi:10.1021/acs.langmuir.7b03311

Designer Neural Networks with Embedded Semiconductor Microtube Arrays

Aune Koitmäe, Manuel Müller, Cornelius S. Bausch, Jann Harberts, Wolfgang Hansen, Gabriele Loers, Robert H. Blick

Research output: Contribution to journal › Article › Research › peer-review

12 Citations (Scopus)

Abstract

Here we present a designer's approach to building cellular neuronal networks based on a biocompatible negative photoresist with embedded coaxial feedthroughs made of semiconductor microtubes. The diameter of the microtubes is tailored and adjusted to the diameter of cerebellum axons having a diameter of 2-3 μm. The microtubes as well as the SU-8 layer serve as a topographical cue to the axons. Apart from the topographical guidance, we also employ chemical guidance cues enhancing neuron growth at designed spots. Therefore, the amino acid poly-l-lysine is printed in droplets of pl volume in the front of the tube entrances. Our artificial neuronal network has an extremely high yield of 85% of the somas settled at the desired locations. We complete this by basic patch-clamp measurements on single cells within the neuronal network.

Original language	English
Pages (from-to)	1528-1534
Number of pages	7
Journal	Langmuir
Volume	34
Issue number	4
DOIs	https://doi.org/10.1021/acs.langmuir.7b03311
Publication status	Published - 30 Jan 2018
Externally published	Yes

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10.1021/acs.langmuir.7b03311

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title = "Designer Neural Networks with Embedded Semiconductor Microtube Arrays",

abstract = "Here we present a designer's approach to building cellular neuronal networks based on a biocompatible negative photoresist with embedded coaxial feedthroughs made of semiconductor microtubes. The diameter of the microtubes is tailored and adjusted to the diameter of cerebellum axons having a diameter of 2-3 μm. The microtubes as well as the SU-8 layer serve as a topographical cue to the axons. Apart from the topographical guidance, we also employ chemical guidance cues enhancing neuron growth at designed spots. Therefore, the amino acid poly-l-lysine is printed in droplets of pl volume in the front of the tube entrances. Our artificial neuronal network has an extremely high yield of 85\% of the somas settled at the desired locations. We complete this by basic patch-clamp measurements on single cells within the neuronal network.",

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AU - Koitmäe, Aune

AU - Müller, Manuel

AU - Bausch, Cornelius S.

AU - Harberts, Jann

AU - Hansen, Wolfgang

AU - Loers, Gabriele

AU - Blick, Robert H.

PY - 2018/1/30

Y1 - 2018/1/30

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AB - Here we present a designer's approach to building cellular neuronal networks based on a biocompatible negative photoresist with embedded coaxial feedthroughs made of semiconductor microtubes. The diameter of the microtubes is tailored and adjusted to the diameter of cerebellum axons having a diameter of 2-3 μm. The microtubes as well as the SU-8 layer serve as a topographical cue to the axons. Apart from the topographical guidance, we also employ chemical guidance cues enhancing neuron growth at designed spots. Therefore, the amino acid poly-l-lysine is printed in droplets of pl volume in the front of the tube entrances. Our artificial neuronal network has an extremely high yield of 85% of the somas settled at the desired locations. We complete this by basic patch-clamp measurements on single cells within the neuronal network.

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Designer Neural Networks with Embedded Semiconductor Microtube Arrays

Abstract

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