Abstract
Macrophages are phagocytic immune cells resident in every tissue that are not only important for host defence, but are also involved in tissue homeostasis, injury, and disease. Despite increasingly sophisticated methods for in vitro macrophage isolation, expansion and activation over the past three decades, these have largely been restricted to modelling bone-marrow or blood-derived cells. The in vitro derivation of macrophages from human pluripotent stem cells provides new opportunities to study macrophage biology, including the factors that impact human myeloid development and those that induce macrophage activation. While sharing many of the functional characteristics of monocyte-derived macrophages, stem cell-derived macrophages may offer new opportunities to understand the role of development or tissue context in innate immune cell function. Immune responsiveness to pathogenic challenge is known to be impacted by a macrophage's history of prior exposure, as well as ontogeny and tissue context. Therefore, we explore the factors of in vitro derivation likely to influence macrophage phenotype and function.
| Original language | English |
|---|---|
| Pages (from-to) | 42-49 |
| Number of pages | 8 |
| Journal | Differentiation |
| Volume | 104 |
| DOIs | |
| Publication status | Published - 1 Nov 2018 |
Keywords
- Innate immunity
- Innate training
- Macrophage culture
- Macrophage polarisation
- Pluripotent stem cell
Cite this
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Designer macrophages : Pitfalls and opportunities for modelling macrophage phenotypes from pluripotent stem cells. / Rajab, Nadia; Rutar, Matthew; Laslett, Andrew L.; Wells, Christine A.
In: Differentiation, Vol. 104, 01.11.2018, p. 42-49.Research output: Contribution to journal › Review Article › Research › peer-review
TY - JOUR
T1 - Designer macrophages
T2 - Pitfalls and opportunities for modelling macrophage phenotypes from pluripotent stem cells
AU - Rajab, Nadia
AU - Rutar, Matthew
AU - Laslett, Andrew L.
AU - Wells, Christine A.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Macrophages are phagocytic immune cells resident in every tissue that are not only important for host defence, but are also involved in tissue homeostasis, injury, and disease. Despite increasingly sophisticated methods for in vitro macrophage isolation, expansion and activation over the past three decades, these have largely been restricted to modelling bone-marrow or blood-derived cells. The in vitro derivation of macrophages from human pluripotent stem cells provides new opportunities to study macrophage biology, including the factors that impact human myeloid development and those that induce macrophage activation. While sharing many of the functional characteristics of monocyte-derived macrophages, stem cell-derived macrophages may offer new opportunities to understand the role of development or tissue context in innate immune cell function. Immune responsiveness to pathogenic challenge is known to be impacted by a macrophage's history of prior exposure, as well as ontogeny and tissue context. Therefore, we explore the factors of in vitro derivation likely to influence macrophage phenotype and function.
AB - Macrophages are phagocytic immune cells resident in every tissue that are not only important for host defence, but are also involved in tissue homeostasis, injury, and disease. Despite increasingly sophisticated methods for in vitro macrophage isolation, expansion and activation over the past three decades, these have largely been restricted to modelling bone-marrow or blood-derived cells. The in vitro derivation of macrophages from human pluripotent stem cells provides new opportunities to study macrophage biology, including the factors that impact human myeloid development and those that induce macrophage activation. While sharing many of the functional characteristics of monocyte-derived macrophages, stem cell-derived macrophages may offer new opportunities to understand the role of development or tissue context in innate immune cell function. Immune responsiveness to pathogenic challenge is known to be impacted by a macrophage's history of prior exposure, as well as ontogeny and tissue context. Therefore, we explore the factors of in vitro derivation likely to influence macrophage phenotype and function.
KW - Innate immunity
KW - Innate training
KW - Macrophage culture
KW - Macrophage polarisation
KW - Pluripotent stem cell
UR - http://www.scopus.com/inward/record.url?scp=85056640653&partnerID=8YFLogxK
U2 - 10.1016/j.diff.2018.10.001
DO - 10.1016/j.diff.2018.10.001
M3 - Review Article
VL - 104
SP - 42
EP - 49
JO - Differentiation
JF - Differentiation
SN - 0301-4681
ER -